Hanicinec Vojtech, Brynychova Veronika, Rosendorf Jachym, Palek Richard, Liska Vaclav, Oliverius Martin, Kala Zdenek, Mohelnikova-Duchonova Beatrice, Krus Ivona, Soucek Pavel
Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic.
Deparment of Surgery, Teaching Hospital and Faculty of Medicine in Pilsen, Charles University, 30460 Pilsen, Czech Republic.
Oncol Lett. 2021 Aug;22(2):598. doi: 10.3892/ol.2021.12859. Epub 2021 Jun 9.
Colorectal cancer is one of the most common cancers and pancreatic cancer is among the most fatal and difficult to treat. New prognostic biomarkers are urgently needed to improve the treatment of colorectal and pancreatic cancer. Protein regulating cytokinesis 1 (), kinesin family member 14 () and citron Rho-interacting serine/threonine kinase () serve important roles in cytokinesis, are strongly associated with cancer progression and have prognostic potential. The present study aimed to investigate the prognostic relevance of the PRC1, KIF14 and CIT genes in colorectal and pancreatic cancer. and transcript expression was assessed by reverse transcription-quantitative PCR in tumors and paired distant unaffected mucosa from 67 patients with colorectal cancer and tumors and paired non-neoplastic control tissues from 48 patients with pancreatic cancer. The extent of transcript dysregulation between tumor and control tissues and between groups of patients divided by main clinical characteristics, namely patients' age and sex, disease stage, localization and grade, was determined. Finally, the associations of transcript levels in tumors with disease-free interval and overall survival time were evaluated. and transcripts were upregulated in tumors compared with control tissues. and levels strongly correlated to each other in both colorectal and pancreatic tumor and control tissues after correction for multiple testing. However, no significant associations were found among the transcript levels of and and disease-free interval or overall survival time. In summary, the present study demonstrated mutual correlation of PRC1, KIF14 and CIT cytokinesis regulators with no clear prognostic value in pancreatic and colorectal cancers. Hence, according to the results of the present study, transcript levels of these genes cannot be clinically exploited as prognostic biomarkers in colorectal or pancreatic cancer patients.
结直肠癌是最常见的癌症之一，而胰腺癌是最致命且最难治疗的癌症之一。迫切需要新的预后生物标志物来改善结直肠癌和胰腺癌的治疗。蛋白质调控胞质分裂1（PRC1）、驱动蛋白家族成员14（KIF14）和西特龙Rho相互作用丝氨酸/苏氨酸激酶（CIT）在胞质分裂中起重要作用，与癌症进展密切相关且具有预后潜力。本研究旨在探讨PRC1、KIF14和CIT基因在结直肠癌和胰腺癌中的预后相关性。通过逆转录定量PCR评估了67例结直肠癌患者肿瘤组织及配对的远处未受影响黏膜组织，以及48例胰腺癌患者肿瘤组织及配对的非肿瘤对照组织中PRC1和KIF14的转录表达。确定了肿瘤组织与对照组织之间以及根据主要临床特征（即患者年龄和性别、疾病分期、定位和分级）划分的患者组之间转录失调的程度。最后，评估了肿瘤中转录水平与无病生存期和总生存时间的关联。与对照组织相比，肿瘤组织中PRC1和KIF14转录本上调。在对多重检验进行校正后，结直肠癌和胰腺癌的肿瘤及对照组织中PRC1和KIF14水平彼此高度相关。然而，未发现PRC1和KIF14转录水平与无病生存期或总生存时间之间存在显著关联。总之，本研究表明PRC1、KIF14和CIT胞质分裂调节因子相互关联，但在胰腺癌和结直肠癌中无明确的预后价值。因此，根据本研究结果，这些基因的转录水平不能在临床中用作结直肠癌或胰腺癌患者的预后生物标志物。
