• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脂质体纳米颗粒包裹的顺铂靶向卵巢癌中CD24阳性细胞的药代动力学评价及抗肿瘤效力

Pharmacokinetic evaluation and antitumor potency of liposomal nanoparticle encapsulated cisplatin targeted to CD24-positive cells in ovarian cancer.

作者信息

Ashihara Keisuke, Terai Yoshito, Tanaka Tomohito, Tanaka Yoshimichi, Fujiwara Satoe, Maeda Kazuya, Tunetoh Satoshi, Sasaki Hiroshi, Hayashi Masami, Ohmichi Masahide

机构信息

Department of Obstetrics and Gynecology, Osaka Medical College, Takatsuki, Osaka 569-8686, Japan.

Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Hyogo 650-0017, Japan.

出版信息

Oncol Lett. 2020 Mar;19(3):1872-1880. doi: 10.3892/ol.2020.11279. Epub 2020 Jan 9.

DOI:10.3892/ol.2020.11279
PMID:32194682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7038920/
Abstract

CD24, which is upregulated in several human malignancies, is related to Epithelial-mesenchymal-transition (EMT) and has characteristics of cancer stem-like cells, especially in cisplatin-resistant ovarian carcinoma cells. Drug delivery systems represent a promising therapeutic approach for diseases with treatment resistance, and the present study investigated a novel CD24-targeted drug delivery system for advanced ovarian carcinoma. We produced liposomal cisplatin with a red fluorescent substance - cyanine 5.5 (GL-CDDP-Cy5.5). In order to target CD24-positive cells, an anti-CD24 monoclonal antibody was modified to the above drug (CD24-GL-CDDP-Cy5.5). Specific uptake of CD24-GL-CDDP-Cy5.5 was confirmed using a therapeutically resistant ovarian cancer cell line, Caov-3 cells. Antitumor effects of CD24-GL-CDDP-Cy5.5 were then evaluated in Caov-3 ×enograft mice. CD24-GL-CDDP-Cy5.5 showed more specific uptake by flow cytometry than GL-CDDP-Cy5.5. In xenograft mice, GL-CDDP-Cy5.5 and CD24-GL-CDDP-Cy5.5 treatment had significantly higher platinum concentration in disseminated tumor cells than cisplatin (P<0.05). Moreover, CD24-GL-CDDP-Cy5.5 suppressed tumor growth and prolonged survival time compared with other treatments. Median survival times of the control, cisplatin, GL-CDDP-Cy5.5 and CD24-GL-CDDP-Cy5.5 groups were 37, 36, 46 and 54 days after inoculation, respectively. Immunohistochemical analysis showed that CD24-GL-CDDP-Cy5.5 treatment, compared with GL-CDDP-Cy5.5, decreased the number of CD24-positive cells and suppressed the EMT phenomenon significantly (P<0.05). The present study demonstrated that CD24-GL-CDDP-Cy5.5, compared with other treatments, improved therapeutic efficacy. The present results suggested the potential for targeting anticancer therapeutics for CD24-positive cells to prevent disease progression.

摘要

CD24在多种人类恶性肿瘤中表达上调，与上皮-间质转化（EMT）相关，具有癌干细胞样特征，尤其是在顺铂耐药的卵巢癌细胞中。药物递送系统是治疗耐药性疾病的一种有前景的治疗方法，本研究针对晚期卵巢癌研究了一种新型的靶向CD24的药物递送系统。我们制备了带有红色荧光物质花青素5.5的脂质体顺铂（GL-CDDP-Cy5.5）。为了靶向CD24阳性细胞，将抗CD24单克隆抗体修饰到上述药物上（CD24-GL-CDDP-Cy5.5）。使用具有治疗抗性的卵巢癌细胞系Caov-3细胞证实了CD24-GL-CDDP-Cy5.5的特异性摄取。然后在Caov-3异种移植小鼠中评估CD24-GL-CDDP-Cy5.5的抗肿瘤作用。通过流式细胞术显示，CD24-GL-CDDP-Cy5.5比GL-CDDP-Cy5.5表现出更特异性的摄取。在异种移植小鼠中，GL-CDDP-Cy5.5和CD24-GL-CDDP-Cy5.5治疗组的播散肿瘤细胞中的铂浓度明显高于顺铂组（P<0.05）。此外，与其他治疗相比，CD24-GL-CDDP-Cy5.5抑制肿瘤生长并延长了生存时间。接种后，对照组、顺铂组、GL-CDDP-Cy5.5组和CD24-GL-CDDP-Cy5.5组的中位生存时间分别为37天、36天、46天和54天。免疫组织化学分析表明，与GL-CDDP-Cy5.5相比，CD24-GL-CDDP-Cy5.5治疗减少了CD24阳性细胞的数量，并显著抑制了EMT现象（P<0.05）。本研究表明，与其他治疗相比，CD24-GL-CDDP-Cy5.5提高了治疗效果。目前的结果表明，靶向CD24阳性细胞的抗癌治疗具有预防疾病进展的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5ad/7038920/0292658068d2/ol-19-03-1872-g10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5ad/7038920/e08612052138/ol-19-03-1872-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5ad/7038920/41b804862406/ol-19-03-1872-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5ad/7038920/ff8b4d6a1753/ol-19-03-1872-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5ad/7038920/0143d9f9e3e6/ol-19-03-1872-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5ad/7038920/0292658068d2/ol-19-03-1872-g10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5ad/7038920/e08612052138/ol-19-03-1872-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5ad/7038920/41b804862406/ol-19-03-1872-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5ad/7038920/ff8b4d6a1753/ol-19-03-1872-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5ad/7038920/0143d9f9e3e6/ol-19-03-1872-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5ad/7038920/0292658068d2/ol-19-03-1872-g10.jpg

相似文献

1
Pharmacokinetic evaluation and antitumor potency of liposomal nanoparticle encapsulated cisplatin targeted to CD24-positive cells in ovarian cancer.脂质体纳米颗粒包裹的顺铂靶向卵巢癌中CD24阳性细胞的药代动力学评价及抗肿瘤效力
Oncol Lett. 2020 Mar;19(3):1872-1880. doi: 10.3892/ol.2020.11279. Epub 2020 Jan 9.
2
CD24 expression is a marker for predicting clinical outcome and regulates the epithelial-mesenchymal transition in ovarian cancer via both the Akt and ERK pathways.CD24表达是预测临床结果的标志物，并通过Akt和ERK途径调节卵巢癌中的上皮-间质转化。
Oncol Rep. 2017 Jun;37(6):3189-3200. doi: 10.3892/or.2017.5583. Epub 2017 Apr 19.
3
Forkhead Box Protein C2 Promotes Epithelial-Mesenchymal Transition, Migration and Invasion in Cisplatin-Resistant Human Ovarian Cancer Cell Line (SKOV3/CDDP).叉头框蛋白C2促进顺铂耐药人卵巢癌细胞系（SKOV3/CDDP）的上皮-间质转化、迁移和侵袭。
Cell Physiol Biochem. 2016;39(3):1098-110. doi: 10.1159/000447818. Epub 2016 Aug 26.
4
Cisplatin-alginate conjugate liposomes for targeted delivery to EGFR-positive ovarian cancer cells.顺铂-海藻酸钠缀合物脂质体用于靶向递送 EGFR 阳性卵巢癌细胞。
Biomaterials. 2014 May;35(14):4297-309. doi: 10.1016/j.biomaterials.2014.01.035. Epub 2014 Feb 22.
5
Chitosan coated polylactic acid nanoparticle-mediated combinatorial delivery of cisplatin and siRNA/Plasmid DNA chemosensitizes cisplatin-resistant human ovarian cancer cells.壳聚糖包被的聚乳酸纳米颗粒介导顺铂与siRNA/质粒DNA的联合递送可使顺铂耐药的人卵巢癌细胞对化疗敏感。
Mol Pharm. 2014 Aug 4;11(8):2720-33. doi: 10.1021/mp500259e. Epub 2014 Jun 20.
6
Activation of the mitogen-activated protein kinase kinase/extracellular signal-regulated kinase pathway overcomes cisplatin resistance in ovarian carcinoma cells.丝裂原活化蛋白激酶激酶/细胞外信号调节激酶通路的激活可克服卵巢癌细胞对顺铂的耐药性。
Int J Gynecol Cancer. 2012 Jul;22(6):922-9. doi: 10.1097/IGC.0b013e31824f0b13.
7
A novel strategy inducing autophagic cell death in Burkitt's lymphoma cells with anti-CD19-targeted liposomal rapamycin.一种新型策略，通过靶向 CD19 的脂质体雷帕霉素诱导伯基特淋巴瘤细胞自噬性细胞死亡。
Blood Cancer J. 2014 Feb 7;4(2):e180. doi: 10.1038/bcj.2014.2.
8
Co-delivery of cisplatin and paclitaxel by folic acid conjugated amphiphilic PEG-PLGA copolymer nanoparticles for the treatment of non-small lung cancer.叶酸共轭两亲性聚乙二醇-聚乳酸-羟基乙酸共聚物纳米粒共递送顺铂和紫杉醇用于治疗非小细胞肺癌
Oncotarget. 2015 Dec 8;6(39):42150-68. doi: 10.18632/oncotarget.6243.
9
Par-4 downregulation confers cisplatin resistance in pancreatic cancer cells via PI3K/Akt pathway-dependent EMT.PAR-4 下调通过 PI3K/Akt 通路依赖性 EMT 赋予胰腺癌细胞顺铂耐药性。
Toxicol Lett. 2014 Jan 3;224(1):7-15. doi: 10.1016/j.toxlet.2013.10.008. Epub 2013 Oct 19.
10
Antitumor efficacy of cisplatin-loaded glycol chitosan nanoparticles in tumor-bearing mice.载顺铂的壳聚糖纳米粒对荷瘤小鼠的抗肿瘤疗效。
J Control Release. 2008 Apr 7;127(1):41-9. doi: 10.1016/j.jconrel.2007.12.014. Epub 2007 Dec 26.

引用本文的文献

1
Future theranostic strategies: emerging ovarian cancer biomarkers to bridge the gap between diagnosis and treatment.未来的诊疗策略：新兴的卵巢癌生物标志物以弥合诊断与治疗之间的差距。
Front Drug Deliv. 2024 Feb 1;4:1339936. doi: 10.3389/fddev.2024.1339936. eCollection 2024.
2
The biological roles of CD24 in ovarian cancer: old story, but new tales.CD24 在卵巢癌中的生物学作用：老故事，新传说。
Front Immunol. 2023 Jun 9;14:1183285. doi: 10.3389/fimmu.2023.1183285. eCollection 2023.
3
Potential of green-synthesized ZnO NPs against human ovarian teratocarcinoma: an in vitro study.

本文引用的文献

1
Snail promotes ovarian cancer progression by recruiting myeloid-derived suppressor cells via CXCR2 ligand upregulation.蜗牛通过上调 CXCR2 配体招募髓源抑制性细胞促进卵巢癌进展。
Nat Commun. 2018 Apr 27;9(1):1685. doi: 10.1038/s41467-018-03966-7.
2
CD24 expression is a marker for predicting clinical outcome and regulates the epithelial-mesenchymal transition in ovarian cancer via both the Akt and ERK pathways.CD24表达是预测临床结果的标志物，并通过Akt和ERK途径调节卵巢癌中的上皮-间质转化。
Oncol Rep. 2017 Jun;37(6):3189-3200. doi: 10.3892/or.2017.5583. Epub 2017 Apr 19.
3
Overexpression of Notch3 and pS6 Is Associated with Poor Prognosis in Human Ovarian Epithelial Cancer.
绿色合成的氧化锌纳米粒子对人卵巢畸胎癌的作用潜力：一项体外研究。
Mol Biol Rep. 2023 May;50(5):4447-4457. doi: 10.1007/s11033-023-08367-8. Epub 2023 Apr 4.
4
CD24 as a Potential Therapeutic Target in Patients with B-Cell Leukemia and Lymphoma: Current Insights.CD24作为B细胞白血病和淋巴瘤患者的潜在治疗靶点：当前见解
Onco Targets Ther. 2022 Nov 18;15:1391-1402. doi: 10.2147/OTT.S366625. eCollection 2022.
5
A Comparative in Silico Analysis of CD24's Prognostic Value in Human and Canine Prostate Cancer.人及犬前列腺癌中CD24预后价值的计算机模拟比较分析
J Pers Med. 2021 Mar 23;11(3):232. doi: 10.3390/jpm11030232.
6
The rediscovery of platinum-based cancer therapy.铂类抗癌疗法的再发现。
Nat Rev Cancer. 2021 Jan;21(1):37-50. doi: 10.1038/s41568-020-00308-y. Epub 2020 Oct 30.
Notch3和pS6的过表达与人类卵巢上皮癌的不良预后相关。
Mediators Inflamm. 2016;2016:5953498. doi: 10.1155/2016/5953498. Epub 2016 Jun 30.
4
Whole-genome characterization of chemoresistant ovarian cancer.耐药性卵巢癌的全基因组特征分析。
Nature. 2015 May 28;521(7553):489-94. doi: 10.1038/nature14410.
5
The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer.上皮-间质转化（EMT）相关蛋白表达可提示卵巢癌患者的转移状态及预后。
J Ovarian Res. 2014 Jul 27;7:76. doi: 10.1186/1757-2215-7-76.
6
Gemcitabine as a molecular targeting agent that blocks the Akt cascade in platinum-resistant ovarian cancer.吉西他滨作为一种分子靶向药物，可阻断铂耐药性卵巢癌中的Akt信号通路。
J Ovarian Res. 2014 Apr 9;7:38. doi: 10.1186/1757-2215-7-38.
7
Cancer statistics, 2013.癌症统计数据，2013 年。
CA Cancer J Clin. 2013 Jan;63(1):11-30. doi: 10.3322/caac.21166. Epub 2013 Jan 17.
8
Targeting anticancer drug delivery to pancreatic cancer cells using a fucose-bound nanoparticle approach.采用岩藻糖结合纳米颗粒方法靶向胰腺癌癌细胞的抗癌药物递送。
PLoS One. 2012;7(7):e39545. doi: 10.1371/journal.pone.0039545. Epub 2012 Jul 11.
9
CD24, COX-2, and p53 in epithelial ovarian cancer and its clinical significance.CD24、COX-2和p53在上皮性卵巢癌中的表达及其临床意义
Front Biosci (Elite Ed). 2012 Jun 1;4(8):2645-51. doi: 10.2741/e580.
10
Non-invasive quantification of white and brown adipose tissues and liver fat content by computed tomography in mice.用 CT 对小鼠的白色和棕色脂肪组织及肝内脂肪含量进行无创定量分析。
PLoS One. 2012;7(5):e37026. doi: 10.1371/journal.pone.0037026. Epub 2012 May 16.