Al-Khafaji Ahmed S K, Pantazi Paschalia, Acha-Sagredo Amelia, Schache Andrew, Risk Janet M, Shaw Richard J, Liloglou Triantafillos
Department of Biology, College of Science, University of Baghdad, Baghdad 10070, Iraq.
Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, Liverpool L7 8TX, UK.
Oncol Lett. 2020 Mar;19(3):2502-2507. doi: 10.3892/ol.2020.11339. Epub 2020 Jan 23.
gene encodes the hepatoma upregulated protein (HURP), a microtubule associated protein regulating mitotic spindle dynamics, which promotes chromosomal congression and alignment during mitosis, with a potential role in tumorigenesis. In the present study, mRNA expression was investigated by reverse transcription-quantitative PCR in oropharyngeal squamous cell carcinoma (OPSCC). Primary OPSCC tumors from 107 patients and 48 adjacent normal tissues, as well as 12 respiratory tract cancer cell lines (9 head and neck squamous cell carcinoma, 2 lung cancer and 1 normal bronchial) were utilised in the present study. mRNA expression levels of were higher in malignant OPSCC tissues compared with in normal mucosa (P<1×10) and significantly associated with sex and smoking status (P<0.0001). Vinorelbine toxicity at half-maximal inhibitory concentration (IC) was measured in the 11 cancer cell lines using an MTT assay. Sensitivity to vinorelbine was significantly correlated with HURP expression (r=0.636; P=0.035). The data indicated that overexpression is frequent in OPSCC tissues and associated with smoking. The correlation between mRNA expression and vinorelbine response suggests that HURP is a potential modulator of vinorelbine response; therefore, it should be explored for its possible predictive value for the efficiency of vinorelbine treatment in this type of cancer.
基因编码肝癌上调蛋白（HURP），一种调节有丝分裂纺锤体动力学的微管相关蛋白，其在有丝分裂期间促进染色体的汇聚和排列，在肿瘤发生中可能发挥作用。在本研究中，通过逆转录定量PCR检测口咽鳞状细胞癌（OPSCC）中的mRNA表达。本研究使用了107例患者的原发性OPSCC肿瘤、48个相邻正常组织以及12种呼吸道癌细胞系（9种头颈部鳞状细胞癌、2种肺癌和1种正常支气管细胞系）。与正常黏膜相比，恶性OPSCC组织中的mRNA表达水平更高（P<1×10），且与性别和吸烟状况显著相关（P<0.0001）。使用MTT法在11种癌细胞系中测量长春瑞滨在半数最大抑制浓度（IC）时的毒性。对长春瑞滨的敏感性与HURP表达显著相关（r=0.636；P=0.035）。数据表明，在OPSCC组织中过表达很常见且与吸烟有关。mRNA表达与长春瑞滨反应之间的相关性表明，HURP是长春瑞滨反应的潜在调节因子；因此，应探索其对这种癌症中长春瑞滨治疗效果的可能预测价值。
