口咽角化型与非角化型鳞状细胞癌中的人乳头瘤病毒（HPV）和爱泼斯坦-巴尔病毒（EBV）
Human papillomavirus (HPV) and Epstein-Barr virus (EBV) in keratinizing versus non- keratinizing squamous cell carcinoma of the oropharynx.
作者信息
Broccolo Francesco, Ciccarese Giulia, Rossi Agostino, Anselmi Luca, Drago Francesco, Toniolo Antonio
机构信息
1Department of Medicine and Surgery (School of Medicine), University of Milano-Bicocca, Monza, Italy.
5Laboratory of Medical Microbiology, University of Milano-Bicocca, Via Cadore 48, 20900 Monza, Italy.
出版信息
Infect Agent Cancer. 2018 Nov 9;13:32. doi: 10.1186/s13027-018-0205-6. eCollection 2018.
BACKGROUND
Oral and oropharyngeal squamous cell carcinomas (OSCC and OPSCC) represent the majority of head and neck squamous cell carcinomas (HNSCC). Human papillomavirus (HPV) is an important etiologic factor together with Epstein-Barr virus (EBV). Little is known on the prevalence of major herpesviruses [EBV, cytomegalovirus (CMV) and HHV-6, - 7 and - 8] in HNSCCs.
METHODS
Fifty-one formalin-fixed paraffin-embedded (FFPE) tissue samples taken at surgery (40 oropharyngeal, 11 oral) were analyzed for 40 HPV genotypes (20 high-risk types), EBV, CMV, HHV-6, - 7 and - 8 by quantitative PCR. Expression of the HPV-induced p16 protein was also investigated by immunohistochemistry (IHC).
RESULTS
In SCC, the prevalence of EBV was significantly higher compared to that of HPV (EBV 51% vs. HPV 19.5%; = 0.005). HPV infection was found in 25% of OPSCC and in none of the OSCC; conversely, higher prevalence of EBV was found in OSCC (72.7%). HPV and EBV co-infection was detected only in 4 (10%) OPSCC. CMV was detected in only two cases, whereas HHV-6, - 7 and - 8 resulted negative. The prevalence of HPV but no EBV was associated with the non-keratinizing SCC type (NKSCC) compared to the keratinizing SCC type (KSCC)(HPV-DNA < 0.005; EBV = 0.054).
CONCLUSIONS
Single HPV or EBV positivity was higher in OSCC than in OPSCC. Other potentially oncogenic herpesvirus types were minimally or not represented.
背景
口腔和口咽鳞状细胞癌（OSCC和OPSCC）占头颈部鳞状细胞癌（HNSCC）的大部分。人乳头瘤病毒（HPV）与爱泼斯坦-巴尔病毒（EBV）一样是重要的病因。关于主要疱疹病毒[EBV、巨细胞病毒（CMV）和HHV-6、-7和-8]在HNSCC中的流行情况知之甚少。
方法
对51份手术时采集的福尔马林固定石蜡包埋（FFPE）组织样本（40份口咽样本，11份口腔样本）进行40种HPV基因型（20种高危型）、EBV、CMV、HHV-6、-7和-8的定量PCR分析。还通过免疫组织化学（IHC）研究HPV诱导的p16蛋白的表达。
结果
在鳞状细胞癌中，EBV的流行率显著高于HPV（EBV 51%对HPV 19.5%；P = 0.005）。在25%的OPSCC中发现HPV感染，而OSCC中未发现；相反，OSCC中EBV的流行率更高（72.7%）。仅在4例（10%）OPSCC中检测到HPV和EBV共感染。仅在2例中检测到CMV，而HHV-6、-7和-8均为阴性。与角化性鳞状细胞癌（KSCC）相比，HPV而非EBV的流行率与非角化性鳞状细胞癌（NKSCC）类型相关（HPV-DNA P < 0.005；EBV = 0.054）。
结论
OSCC中单一HPV或EBV阳性率高于OPSCC。其他潜在致癌性疱疹病毒类型极少或未出现。
Zotero 插件