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用于芳香族氨基醇生物催化合成在线反应监测的台式核磁共振仪

Benchtop NMR for Online Reaction Monitoring of the Biocatalytic Synthesis of Aromatic Amino Alcohols.

作者信息

Claaßen C, Mack K, Rother D

机构信息

Institute of Bio- and Geosciences - Biotechnology (IBG-1) Forschungszentrum Jülich GmbH 52425 Jülich Germany.

Aachen Biology and Biotechnology (ABBt) RWTH Aachen University 52074 Aachen Germany.

出版信息

ChemCatChem. 2020 Feb 20;12(4):1190-1199. doi: 10.1002/cctc.201901910. Epub 2020 Jan 20.

DOI:10.1002/cctc.201901910
PMID:32194875
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7074048/
Abstract

Online analytics provides insights into the progress of an ongoing reaction without the need for extensive sampling and offline analysis. In this study, we investigated benchtop NMR as an online reaction monitoring tool for complex enzyme cascade reactions. Online NMR was used to monitor a two-step cascade beginning with an aromatic aldehyde and leading to an aromatic amino alcohol as the final product, applying two different enzymes and a variety of co-substrates and intermediates. Benchtop NMR enabled the concentration of the reaction components to be detected in buffered systems in the single-digit mM range without using deuterated solvent. The concentrations determined via NMR were correlated with offline samples analyzed via uHPLC and displayed a good correlation between the two methods. In summary, benchtop NMR proved to be a sensitive, selective and reliable method for online reaction monitoring in (multi-step) biosynthesis. In future, online analytic systems such as the benchtop NMR devices described might not only enable direct monitoring of the reaction, but may also form the basis for self-regulation in biocatalytic reactions.

摘要

在线分析能够深入了解正在进行的反应进程，而无需进行大量取样和离线分析。在本研究中，我们研究了台式核磁共振仪作为复杂酶级联反应的在线反应监测工具。在线核磁共振被用于监测一个两步级联反应，该反应从一种芳香醛开始，最终生成芳香氨基醇，使用了两种不同的酶以及多种共底物和中间体。台式核磁共振仪能够在不使用氘代溶剂的情况下，在缓冲体系中检测到浓度处于个位数毫摩尔范围内的反应组分。通过核磁共振测定的浓度与通过超高效液相色谱分析的离线样品相关，并且两种方法之间显示出良好的相关性。总之，台式核磁共振被证明是一种用于（多步）生物合成中在线反应监测的灵敏、选择性好且可靠的方法。未来，诸如本文所述的台式核磁共振仪之类的在线分析系统可能不仅能够直接监测反应，还可能成为生物催化反应中自我调节的基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd8c/7074048/a0d6acc14008/CCTC-12-1190-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd8c/7074048/1dd907f852e2/CCTC-12-1190-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd8c/7074048/a0d6acc14008/CCTC-12-1190-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd8c/7074048/acd63c0d5d14/CCTC-12-1190-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd8c/7074048/9494f7f4c11a/CCTC-12-1190-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd8c/7074048/228b6eaa3fe2/CCTC-12-1190-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd8c/7074048/a0d6acc14008/CCTC-12-1190-g007.jpg

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本文引用的文献

1
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Biotechnol Bioeng. 2019 Nov;116(11):2874-2883. doi: 10.1002/bit.27112. Epub 2019 Jul 21.
2
Stimulus-Responsive Regulation of Enzyme Activity for One-Step and Multi-Step Syntheses.用于一步和多步合成的酶活性的刺激响应调节
Adv Synth Catal. 2019 Jun 6;361(11):2387-2401. doi: 10.1002/adsc.201900169. Epub 2019 Apr 15.
3
Benchtop NMR for the monitoring of bioprocesses.
Chemistry. 2022 Mar 1;28(13):e202103607. doi: 10.1002/chem.202103607. Epub 2022 Jan 10.
4
Modulation of Transaminase Activity by Encapsulation in Temperature-Sensitive Poly(N-acryloyl glycinamide) Hydrogels.温度敏感型聚（N-丙烯酰基甘氨酸酰胺）水凝胶对转氨酶活性的调节作用。
Chembiochem. 2021 Dec 10;22(24):3452-3461. doi: 10.1002/cbic.202100427. Epub 2021 Oct 13.
台式 NMR 用于生物工艺监测。
Magn Reson Chem. 2019 Aug;57(10):794-804. doi: 10.1002/mrc.4821. Epub 2019 Jan 22.
4
Highly efficient production of chiral amines in batch and continuous flow by immobilized ω-transaminases on controlled porosity glass metal-ion affinity carrier.在控制孔玻璃金属离子亲和载体上固定化ω-转氨酶，在分批和连续流中高效生产手性胺。
J Biotechnol. 2019 Feb 10;291:52-60. doi: 10.1016/j.jbiotec.2018.12.001. Epub 2018 Dec 11.
5
An Autonomous Self-Optimizing Flow Reactor for the Synthesis of Natural Product Carpanone.一种用于天然产物 Carpanone 合成的自主自优化流反应器。
J Org Chem. 2018 Dec 7;83(23):14286-14299. doi: 10.1021/acs.joc.8b01821. Epub 2018 Oct 3.
6
Statistical optimisation of enzymatic detoxification with laccase from Trametes versicolor for spent sulphite liquors using a novel in-situ NMR method.用新型原位 NMR 方法对硫磺基废液进行酶解毒，优化彩绒革盖菌漆酶的酶解工艺。
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7
Online low-field NMR spectroscopy for process control of an industrial lithiation reaction-automated data analysis.在线低场 NMR 光谱法用于工业锂化反应过程控制-自动化数据分析。
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8
Selective aerobic oxidation reactions using a combination of photocatalytic water oxidation and enzymatic oxyfunctionalisations.使用光催化水氧化和酶促氧官能化相结合的选择性好氧氧化反应。
Nat Catal. 2018 Jan;1(1):55-62. doi: 10.1038/s41929-017-0001-5. Epub 2017 Nov 20.
9
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Angew Chem Int Ed Engl. 2018 Jun 11;57(24):6996-7010. doi: 10.1002/anie.201707084. Epub 2017 Dec 12.
10
The Application of Quantitative ¹H-NMR for the Determination of Orlistat in Tablets.定量 ¹H-NMR 在片剂中奥利司他测定的应用。
Molecules. 2017 Sep 10;22(9):1517. doi: 10.3390/molecules22091517.