Claaßen Christiane, Gerlach Tim, Rother Dörte
Institute of Bio- and Geosciences - Biotechnology (IBG-1) Forschungszentrum Jülich GmbH 52425 Jülich Germany.
Aachen Biology and Biotechnology (ABBt) RWTH Aachen University 52074 Aachen Germany.
Adv Synth Catal. 2019 Jun 6;361(11):2387-2401. doi: 10.1002/adsc.201900169. Epub 2019 Apr 15.
Multi-step biocatalytic reactions have gained increasing importance in recent years because the combination of different enzymes enables the synthesis of a broad variety of industrially relevant products. However, the more enzymes combined, the more crucial it is to avoid cross-reactivity in these cascade reactions and thus achieve high product yields and high purities. The selective control of enzyme activity, i.e., remote on-/off-switching of enzymes, might be a suitable tool to avoid the formation of unwanted by-products in multi-enzyme reactions. This review compiles a range of methods that are known to modulate enzyme activity in a stimulus-responsive manner. It focuses predominantly on systems and is subdivided into reversible and irreversible enzyme activity control. Furthermore, a discussion section provides indications as to which factors should be considered when designing and choosing activity control systems for biocatalysis. Finally, an outlook is given regarding the future prospects of the field.
近年来，多步生物催化反应变得越来越重要，因为不同酶的组合能够合成各种各样与工业相关的产品。然而，组合的酶越多，在这些级联反应中避免交叉反应从而实现高产品收率和高纯度就越关键。酶活性的选择性控制，即酶的远程开启/关闭，可能是避免在多酶反应中形成不需要的副产物的合适工具。本综述汇编了一系列已知的以刺激响应方式调节酶活性的方法。它主要关注[此处原文似乎缺失相关内容]系统，并细分为可逆和不可逆的酶活性控制。此外，讨论部分指出了在设计和选择用于生物催化的活性控制系统时应考虑哪些因素。最后，对该领域的未来前景进行了展望。
