Shaw James, Gary Joshua, Baker Andrea, Kaplan Heidi, Kim Yesul, Milhoan Madison, Burgess Andrew, Ambrose Catherine
McGovern Medical School, The University of Texas Health and Science Center, Houston, Texas.
J Orthop Trauma. 2020 Apr;34(4):e109-e113. doi: 10.1097/BOT.0000000000001729.
Prefabrication and storage of antibiotic beads may decrease surgical time and allow for use in other settings. This study investigated the effects of sterilization technique and storage time on the bioactivity of antibiotic polymethyl methacrylate (PMMA) beads.
Uniform beads of PMMA containing 1 g each of tobramycin and vancomycin were sterilized using autoclave, ethylene oxide (ETO), or ultraviolet (UV) light. Beads were made in a sterile fashion as a control. Disks containing eluted antibiotics from each of the 4 groups were placed onto agar plates inoculated with Staphylococcus aureus. Zones of inhibition, a measure of bioactivity for antibiotic eluted, were determined for the experimental groups and control. Repeat testing was performed for beads stored for 1 week, 1, 3, and 6 months.
Beads sterilized using autoclave, ETO, and UV light showed similar ZOIs after 24 hours of and 1 week of elution compared with the control group. Beads stored for up to 6 months demonstrated similar bioactivity to beads made sterile and tested immediately.
PMMA beads containing vancomycin and tobramycin made in a sterile fashion and stored for up to 6 months have the same efficacy as the current standard of beads made sterile and used immediately. The elution and bioactivity of vancomycin-tobramycin antibiotic beads are not negatively impacted by the sterilization methods of autoclaving, ETO gas, or UV light. Furthermore, nonsterile beads can be sterilized and stored up to 6 months with an expected efficacy comparable with beads made in a standard sterile fashion.
抗生素珠粒的预制和储存可能会减少手术时间，并可用于其他情况。本研究调查了灭菌技术和储存时间对抗生素聚甲基丙烯酸甲酯（PMMA）珠粒生物活性的影响。
将含有1克妥布霉素和万古霉素的均匀PMMA珠粒分别采用高压灭菌、环氧乙烷（ETO）或紫外线（UV）进行灭菌。以无菌方式制作珠粒作为对照。将来自4组中每组洗脱抗生素的圆盘置于接种有金黄色葡萄球菌的琼脂平板上。测定实验组和对照组的抑菌圈，作为抗生素洗脱生物活性的指标。对储存1周、1个月、3个月和6个月的珠粒进行重复测试。
与对照组相比，采用高压灭菌、ETO和紫外线灭菌的珠粒在洗脱24小时和1周后显示出相似的抑菌圈。储存长达6个月的珠粒显示出与无菌制作并立即测试的珠粒相似的生物活性。
以无菌方式制作并储存长达6个月的含万古霉素和妥布霉素的PMMA珠粒与目前无菌制作并立即使用的标准珠粒具有相同的疗效。万古霉素-妥布霉素抗生素珠粒的洗脱和生物活性不受高压灭菌、ETO气体或紫外线灭菌方法的负面影响。此外，非无菌珠粒可以进行灭菌并储存长达6个月，预期疗效与以标准无菌方式制作的珠粒相当。
