Zheng J-P, Dai Y-M, Chen Z, Chen Q, Zheng Y, Lin X, Cui T-J
Department of Oncology, Fujian Provincial Hospital, Fuzhou, China.
Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2518-2524. doi: 10.26355/eurrev_202003_20519.
Recent researches have proved that circular RNAs (circRNAs) act as an important role in many diseases. Our study aims to uncover the role of circ-ABCB10 in the progression of non-small cell lung cancer (NSCLC).
Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was utilized to detect circ-ABCB10 expression in NSCLC patients. Then, we conducted Cell Counting Kit-8 (CCK-8) assay, colony formation assay, Ethynyl deoxyuridine (EdU) incorporation assay, cell cycle assay, and cell apoptosis assay in treated NSCLC cells. Besides, further experiments including RT-qPCR and Western blot assay were performed to explore the potential mechanism in vitro.
Circ-ABCB10 expression level was significantly higher in NSCLC samples comparing to that in adjacent tissues. Moreover, functional assays showed that the cell growth ability of NSCLC cells was inhibited after circ-ABCB10 was knocked down. In addition, the cell apoptosis of NSCLC cells was promoted after circ-ABCB10 was knocked down. Also the expression of KISS1 was upregulated by the knockdown of circ-ABCB10. Furthermore, it was found that KISS1 expression was negatively correlated to the circ-ABCB10 expression in NSCLC tissues.
Results above indicated that circ-ABCB10 promoted cell proliferation and inhibited cell apoptosis of NSCLC by suppressing KISS1, which suggested that circ-ABCB10 may be a potential therapeutic target in NSCLC.
近期研究已证明环状RNA（circRNAs）在多种疾病中发挥重要作用。本研究旨在揭示circ-ABCB10在非小细胞肺癌（NSCLC）进展中的作用。
采用实时定量聚合酶链反应（RT-qPCR）检测NSCLC患者中circ-ABCB10的表达。然后，我们对处理后的NSCLC细胞进行了细胞计数试剂盒-8（CCK-8）检测、集落形成检测、乙炔基脱氧尿苷（EdU）掺入检测、细胞周期检测和细胞凋亡检测。此外，还进行了包括RT-qPCR和蛋白质免疫印迹检测在内的进一步实验以在体外探索潜在机制。
与癌旁组织相比，NSCLC样本中circ-ABCB10表达水平显著更高。此外，功能检测表明敲低circ-ABCB10后NSCLC细胞的生长能力受到抑制。另外，敲低circ-ABCB10后NSCLC细胞的凋亡增加。而且敲低circ-ABCB10后KISS1的表达上调。此外，发现在NSCLC组织中KISS1表达与circ-ABCB10表达呈负相关。
上述结果表明circ-ABCB10通过抑制KISS1促进NSCLC细胞增殖并抑制细胞凋亡，这提示circ-ABCB10可能是NSCLC的一个潜在治疗靶点。
