Department of Mammary and Thyroid Surgery, Weihai Central Hospital, Weihai, China.
Eur Rev Med Pharmacol Sci. 2020 Feb;24(3):1271-1277. doi: 10.26355/eurrev_202002_20182.
Recent researches have proved that circular RNAs (circRNAs) play important roles in many diseases. Thyroid cancer is one of the most common malignant tumors worldwide. Therefore, the aim of this study was to investigate the role of circ-ABCB10 in thyroid cancer.
Circ-ABCB10 expression in 40 paired thyroid cancer tissues and adjacent normal tissues was monitored by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). Subsequently, circ-ABCB10 was silenced or overexpressed in thyroid cancer cells. Function assays were conducted to explore the role of circ-ABCB10 in the proliferation and invasion of thyroid cancer in vitro. Furthermore, RT-qPCR and Western blot assay were performed to elucidate the potential underlying mechanism.
Circ-ABCB10 expression was significantly higher in thyroid cancer tissues than that in adjacent tissues. The growth ability of thyroid cancer cells was significantly inhibited after circ-ABCB10 silence. However, the growth ability of thyroid cancer cells was remarkably promoted after circ-ABCB10 was overexpressed in vitro. Similarly, the invasion of thyroid cancer cells was significantly inhibited or promoted after circ-ABCB10 silence or overexpression, respectively. Besides, the expression of KLF6 was markedly up-regulated by the silence of circ-ABCB10. However, KLF6 expression was down-regulated by overexpression of circ-ABCB10.
Circ-ABCB10 was first identified as a novel oncogene in thyroid cancer. Furthermore, it significantly enhanced the proliferation and invasion of thyroid cancer by targeting KLF6. Our findings suggested that circ-ABCB10 could be used as a potential therapeutic target in thyroid cancer.
最近的研究证明，环状 RNA（circRNAs）在许多疾病中发挥重要作用。甲状腺癌是全球最常见的恶性肿瘤之一。因此，本研究旨在探讨 circ-ABCB10 在甲状腺癌中的作用。
通过实时定量聚合酶链反应（RT-qPCR）监测 40 对甲状腺癌组织和相邻正常组织中的 circ-ABCB10 表达。随后，在甲状腺癌细胞中沉默或过表达 circ-ABCB10。进行功能测定以探讨 circ-ABCB10 在甲状腺癌细胞体外增殖和侵袭中的作用。此外，进行 RT-qPCR 和 Western blot 分析以阐明潜在的机制。
circ-ABCB10 在甲状腺癌组织中的表达明显高于相邻组织。沉默 circ-ABCB10 后，甲状腺癌细胞的生长能力明显受到抑制。然而，体外过表达 circ-ABCB10 后，甲状腺癌细胞的生长能力显著增强。同样，沉默或过表达 circ-ABCB10 后，甲状腺癌细胞的侵袭能力分别显著受到抑制或促进。此外，沉默 circ-ABCB10 可显著上调 KLF6 的表达。然而，过表达 circ-ABCB10 可下调 KLF6 的表达。
circ-ABCB10 首次被鉴定为甲状腺癌中的一种新型癌基因。此外，它通过靶向 KLF6 显著增强了甲状腺癌细胞的增殖和侵袭能力。我们的研究结果表明，circ-ABCB10 可作为甲状腺癌的潜在治疗靶点。
