Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China.
Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2564-2571. doi: 10.26355/eurrev_202003_20525.
The purpose of this study was to analyze the relationship between von Willebrand factor (vWF) expression and lymph node metastasis or hemodynamics parameters in PTC. This work will provide a novel biomarker for the diagnosis of papillary thyroid carcinoma (PTC).
A total of 156 PTC patients were divided into metastatic and non-metastatic groups based on the presence or absence of lymph node metastasis. The Adler blood flow grading, color doppler flow imaging (CDFI), and blood flow index (PSV, PI, RI, AT) were measured and analyzed between the two groups. The expression of vWF was examined by immunocytochemical assay and quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). The function of vWF was investigated by methyl thiazolyl tetrazolium (MTT) and the transwell assays.
Both metastatic and non-metastatic groups with the major Adler grades as 0-1 had abundant blood flows. There was a significant difference in the rate of lymph node metastasis between Adler 2-3 and Adler 0-1. Moreover, the expression of vWF was found to be associated with lymph node metastasis or Adler blood flow grade in PTC. Significant differences in peak systolic velocity (PSV), systolic acceleration time (AT), and resistance index (RI) were detected in metastatic and non-metastatic groups. In addition, the upregulation of vWF was positively correlated with PSV, RI, and PI in PTC. Functionally, the knockdown of vWF inhibited the development of PTC by suppressing cell proliferation, migration, and invasion.
Abnormal expression of vWF is closely related to lymph node metastasis and hemodynamics parameters in PTC patients. Furthermore, vWF plays an oncogene role in PTC progression.
本研究旨在分析血管性血友病因子（vWF）表达与 PTC 淋巴结转移或血液动力学参数之间的关系。这项工作将为甲状腺乳头状癌（PTC）的诊断提供一种新的生物标志物。
根据有无淋巴结转移，将 156 例 PTC 患者分为转移组和非转移组。测量并分析两组间 Adler 血流分级、彩色多普勒血流成像（CDFI）和血流指数（PSV、PI、RI、AT）。采用免疫细胞化学法和实时定量聚合酶链反应（qRT-PCR）检测 vWF 的表达。通过甲基噻唑基四唑（MTT）和 Transwell 检测评估 vWF 的功能。
转移性和非转移性组中 Adler 分级主要为 0-1 的两组均有丰富的血流。Adler 2-3 与 Adler 0-1 之间的淋巴结转移率存在显著差异。此外，vWF 的表达与 PTC 的淋巴结转移或 Adler 血流分级有关。转移性和非转移性组间收缩期峰值速度（PSV）、收缩期加速度时间（AT）和阻力指数（RI）存在显著差异。此外，在 PTC 中，vWF 的上调与 PSV、RI 和 PI 呈正相关。功能上，vWF 的敲低通过抑制细胞增殖、迁移和侵袭抑制了 PTC 的发展。
vWF 的异常表达与 PTC 患者的淋巴结转移和血液动力学参数密切相关。此外，vWF 在 PTC 进展中发挥癌基因作用。
