Guo Shu, Liu Li, Li Ying, Shi Hao, Cong A-Ni
Department of Obstetrics and Gynecology, Dalian Municipal Central Hospital, Dalian, China.
Department of Ultrasound, Chongqing Key Laboratory for Intelligent Oncology in Breast Cancer (iCQBC)·Chongqing University Cancer Hospital, Chongqing, China.
Transl Cancer Res. 2022 May;11(5):1354-1361. doi: 10.21037/tcr-22-1114.
This study aimed to explore the correlation between nm23-H_1 gene expression and transvaginal color Doppler ultrasound performance following radiotherapy (RT) for cervical cancer.
Eighty patients with cervical cancer treated surgically after RT were retrospectively included in this study. Another 60 normal cervical tissue specimens from the pathology department and 30 cervical cancer tissues that did not undergo preoperative RT were included as control samples. Nm23-H_1 protein expression in cervical tissue was detected using immunohistochemistry. Color Doppler flow imaging (CDFI) was used to detect the imaging signs and resistance index (RI) of cervical cancer.
(I) Immunohistochemical detection showed that the positive rates of the nm23-H_1 protein in cervical cancer and normal cervical tissues were 37.5% (30/80) and 70.0% (42/60), respectively, and the difference was statistically significant (χ=14.498, P=0.000). In cervical cancer cells, nm23-H_1 was mainly localized in the cytoplasm but was also expressed in small amounts in the cell membrane. (II) We observed that the expression status of nm23-H_1 was closely correlated with the tumor diameter, lymph node metastasis status, and differentiation degree of patients (P0.05). The positive rate of nm23-H_1 was higher in patients with cervical cancer with larger tumor diameters, presence of lymph node metastasis, and lower differentiation degree. (III) Ultrasound findings in the 80 cervical cancer patients exhibited no echogenicity in two cases, moderate echogenicity in six cases, hypoechogenicity in 36 cases, and hyperechogenicity in 36 cases. The maximum RI of the patients' cervical tumors was 0.612, the minimum RI was 0.273, and the mean RI was 0.45. Nm23-H_1 protein expression was significantly correlated with CDFI grade and RI (P<0.05). Patients with positive nm23-H_1 expression had a significantly higher CDFI grade and RI compared to patients in the nm23-H_1 negative expression group.
Nm23-H_1 protein expression following RT for cervical cancer was significantly correlated with the clinicopathological parameters, CDFI grading, and RI index of cervical cancer patients.
本研究旨在探讨宫颈癌放疗（RT）后nm23-H_1基因表达与经阴道彩色多普勒超声表现之间的相关性。
本研究回顾性纳入了80例放疗后接受手术治疗的宫颈癌患者。另外，纳入病理科60例正常宫颈组织标本和30例未接受术前放疗的宫颈癌组织作为对照样本。采用免疫组织化学法检测宫颈组织中nm23-H_1蛋白表达。采用彩色多普勒血流成像（CDFI）检测宫颈癌的影像学征象及阻力指数（RI）。
（I）免疫组织化学检测显示，宫颈癌组织和正常宫颈组织中nm23-H_1蛋白的阳性率分别为37.5%（30/80）和70.0%（42/60），差异有统计学意义（χ=14.498，P=0.000）。在宫颈癌细胞中，nm23-H_1主要定位于细胞质，但在细胞膜中也有少量表达。（II）我们观察到nm23-H_1的表达状态与患者的肿瘤直径、淋巴结转移状态及分化程度密切相关（P<0.05）。肿瘤直径较大、存在淋巴结转移及分化程度较低的宫颈癌患者中nm23-H_1的阳性率较高。（III）80例宫颈癌患者的超声表现中，2例无回声，6例中等回声，36例低回声，36例高回声。患者宫颈肿瘤的最大RI为0.612，最小RI为0.273，平均RI为0.45。nm23-H_1蛋白表达与CDFI分级及RI显著相关（P<0.05）。与nm23-H_1阴性表达组患者相比，nm23-H_1阳性表达患者的CDFI分级和RI显著更高。
宫颈癌放疗后nm23-H_1蛋白表达与宫颈癌患者的临床病理参数、CDFI分级及RI指数显著相关。
