Department of Nuclear Medicine, Qingdao Municipal Hospital, Qingdao, China.
Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2572-2578. doi: 10.26355/eurrev_202003_20526.
The aim of this study was to examine the regulatory role of circ-0103552 in the procession of thyroid carcinoma (TC) and the related underlying mechanisms.
The tissues were obtained from 56 patients diagnosed with TC in our hospital. Nthy-ori3-1 cell line and TC cell lines (TPC-1, SW579, 8505C) were purchased from American Type Culture Collection (ATCC). Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was conducted to analyze the expression of circ-0103552 in TC tissues and cell lines. Inhibition of circ-0103552 was achieved by circ-0103552 siRNA. Dual-Luciferase report assay was performed to confirm the potential target miRNA of circ-0103552. The transwell assay and wound-healing assay were designed to examine the invasion and migration ability of TC cells, respectively.
Circ-0103552 was detected to be upregulated in TC tissues, as well as in TC cell lines, including TPC-1, SW579, and 8505C. The knockdown of circ-0103552 significantly reduced the invasion and migration ability in TC cells. It was predicted using the circular RNA database that microRNA-127 (miR-127) was a target miRNA of circ-0103552, which was confirmed by the Dual-Luciferase assay. Further studies revealed that circ-0103552 was involved in the invasion and migration of TC by sponging miR-127.
The present study demonstrated that circ-0103552 acts as a regulator in the invasion and migration of TC by sponging miR-127.
本研究旨在探讨 circ-0103552 在甲状腺癌（TC）发生发展中的调控作用及其相关机制。
收集我院 56 例 TC 患者的组织标本。Nthy-ori3-1 细胞系和 TC 细胞系（TPC-1、SW579、8505C）购自美国典型培养物保藏中心（ATCC）。采用实时定量聚合酶链反应（qRT-PCR）分析 TC 组织和细胞系中 circ-0103552 的表达。采用 circ-0103552 siRNA 抑制 circ-0103552 的表达。采用双荧光素酶报告实验验证 circ-0103552 的潜在靶 miRNA。通过 Transwell 实验和划痕愈合实验分别检测 TC 细胞的侵袭和迁移能力。
circ-0103552 在 TC 组织和 TPC-1、SW579、8505C 等 TC 细胞系中均呈高表达。circ-0103552 下调显著抑制 TC 细胞的侵袭和迁移能力。CircRNA 数据库预测 miR-127 是 circ-0103552 的靶 miRNA，双荧光素酶实验进一步证实了这一预测。进一步研究表明，circ-0103552 通过海绵吸附 miR-127 参与 TC 的侵袭和迁移。
本研究表明，circ-0103552 通过海绵吸附 miR-127 调控 TC 的侵袭和迁移。
