Department of Health Management, Jeonju University, Jeonju-si, Jeollabuk-do 55069, Republic of Korea; Department of Food Science and Technology, Chonbuk National University, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.
Department of Health Management, Jeonju University, Jeonju-si, Jeollabuk-do 55069, Republic of Korea.
Int Immunopharmacol. 2020 Jun;83:106403. doi: 10.1016/j.intimp.2020.106403. Epub 2020 Mar 18.
IL-31 and IL-33 are cytokines, which are expressed in many inflammatory and pathological disorders, thus suggesting an IL-31/IL-33 axis interaction in pathological diseases. Luteolin from natural products is known for its anti-inflammatory activities associated with the regulation of inflammatory signaling pathways. Here, we investigated the effects of luteolin in the regulation of IL-33-stimulated production and secretion of IL-31 in HMC-1.2 mast cells. Human mast cells (HMC-1.2) were treated with luteolin and stimulated with IL-33. Real-time PCR was used to measure IL-31 mRNA expression. Western blot and immunofluorescence assays were used to measure IL-31 expression. ELISA techniques were used to measure IL-31 secretion and NF-κB-DNA-binding activities. The results revealed that luteolin inhibited the expression of IL-31 in IL-33-stimulated HMC-1.2 cells at the mRNA and protein levels. Also, Luteolin inhibited the secretion of IL-31 into the cell culture media of the IL-33-stimulated HMC-1.2 cells. Further findings demonstrated that luteolin inhibited the activation of ERK, JNK, p38, and NF-κB p65 in the IL-33-stimulated HMC-1.2 cells. In addition, luteolin also prevented the nuclear translocation and binding of p65 to its DNA-binding site. Based on the results, luteolin may be considered as a potential therapeutic or functional food agent for the prevention and/or treatment of IL-31 and IL-33-related diseases.
IL-31 和 IL-33 是细胞因子，它们在许多炎症和病理疾病中表达，因此提示 IL-31/IL-33 轴在病理疾病中相互作用。天然产物中的木犀草素以其与炎症信号通路调节相关的抗炎活性而闻名。在这里，我们研究了木犀草素在调节 HMC-1.2 肥大细胞中 IL-33 刺激的 IL-31 产生和分泌中的作用。用木犀草素处理人肥大细胞 (HMC-1.2) 并刺激其产生 IL-33。实时 PCR 用于测量 IL-31 mRNA 表达。Western blot 和免疫荧光测定用于测量 IL-31 表达。ELISA 技术用于测量 IL-31 分泌和 NF-κB-DNA 结合活性。结果表明，木犀草素在 IL-33 刺激的 HMC-1.2 细胞中抑制 IL-31 在 mRNA 和蛋白质水平上的表达。此外，木犀草素抑制 IL-33 刺激的 HMC-1.2 细胞中 IL-31 分泌到细胞培养物中的分泌。进一步的研究结果表明，木犀草素抑制了 IL-33 刺激的 HMC-1.2 细胞中 ERK、JNK、p38 和 NF-κB p65 的激活。此外，木犀草素还阻止了 p65 的核易位和与 DNA 结合位点的结合。基于这些结果，木犀草素可被视为预防和/或治疗与 IL-31 和 IL-33 相关疾病的潜在治疗或功能性食品剂。
