School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, 100 Waihuanxi Road, Guangzhou, 510006, PR China.
School of Computers, Guangdong University of Technology, 100 Waihuanxi Road, Guangzhou, 510006, PR China.
Neurotox Res. 2021 Dec;39(6):1800-1811. doi: 10.1007/s12640-021-00426-x. Epub 2021 Oct 16.
Microglia-mediated neuroinflammation in response to injurious self and non-self-stimuli exerts detrimental effects on neurons, which may lead to cognitive impairment. Luteolin, a typical kind of natural flavonoid in honeysuckle, chrysanthemum, and Herba Schizonepetae, is widely recognized to be anti-inflammatory and antioxidant against peripheral inflammation. However, its protective effect against inflammation-induced cognitive impairment is currently unknown. In this paper, we investigated the relief potential of luteolin against lipopolysaccharide (LPS)-induced cognitive impairment and neuroinflammation and its possible anti-inflammatory mechanisms in lipopolysaccharide-stimulated BV2 microglia cells. In this study, luteolin ameliorated LPS-induced cognitive impairments, indicated by behavioral performance of neuroinflammatory model mice in Morris water maze tests. Protein analyses and histological examination also revealed protective effect of luteolin against neuronal damage, through inhibiting overproduction of inflammatory cytokines in both hippocampus and cortex of mice. We also observed luteolin in vitro significantly suppressed the levels of pro-inflammatory cytokines, such as tumor necrosis factor alpha (TNF-α) and interleukin-1 β (IL-1β), and inflammatory mediators like nitric oxide. Taken together, these results demonstrated luteolin was effective in alleviating cognitive impairment and limited neuronal damage via inhibiting the release of inflammatory mediators, suggesting luteolin is potential for further therapeutic research of neuroinflammation-related neurodegenerative diseases.
小胶质细胞介导的对损伤性自身和非自身刺激的神经炎症对神经元产生有害影响，可能导致认知障碍。木犀草素是金银花、菊花和荆芥中的一种典型天然类黄酮，被广泛认为具有抗炎和抗氧化作用，可抵抗外周炎症。然而，其对炎症引起的认知障碍的保护作用目前尚不清楚。在本文中，我们研究了木犀草素对脂多糖 (LPS) 诱导的认知障碍和神经炎症的缓解潜力，以及其在 LPS 刺激的 BV2 小胶质细胞中可能的抗炎机制。在这项研究中，木犀草素改善了 LPS 诱导的认知障碍，表现在神经炎症模型小鼠在 Morris 水迷宫测试中的行为表现上。蛋白分析和组织学检查还表明，木犀草素通过抑制小鼠海马和皮质中炎症细胞因子的过度产生，对神经元损伤具有保护作用。我们还观察到木犀草素在体外显著抑制了促炎细胞因子（如肿瘤坏死因子-α (TNF-α) 和白细胞介素-1β (IL-1β)）和炎症介质（如一氧化氮）的水平。总之，这些结果表明木犀草素通过抑制炎症介质的释放，有效缓解认知障碍和限制神经元损伤，表明木犀草素可能是神经炎症相关神经退行性疾病进一步治疗研究的候选药物。
