Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, India; Department of Chemical Sciences, Tezpur University, Tezpur 784028, India.
Department of Chemical Sciences, Tezpur University, Tezpur 784028, India.
Int J Biol Macromol. 2020 Jul 1;154:672-682. doi: 10.1016/j.ijbiomac.2020.03.158. Epub 2020 Mar 17.
In this study, microcrystalline cellulose (MCC) was isolated from Saccharum spontaneum by integrating alkaline delignification, chlorine-free bleaching, and acid hydrolysis treatments, through an environment friendly and sustainable method. To minimize acid concentrations, the acid hydrolysis conditions were optimized using Taguchi orthogonal L design that evaluated the influences of reaction time, temperature, acid concentration and solution to pulp ratio on the physical and chemical characteristics of MCC. The cellulose source at its different stages of processing was submitted to various analytical techniques for morphological and physiochemical investigations. The highest MCC yield optimized was 83%. This process is favorable due to the use of very low (5% HSO) acid concentration, low corrosivity, effluent reduction, and cost-effectiveness. Detailed analyses showed that the isolated MCC has good crystallinity and thermal stability and hence expected as a high-value precursor for the production of polymer biocomposites for diverse applications.
在这项研究中，通过整合碱性脱木质素、无氯漂白和酸水解处理，从甜根子草中分离出微晶纤维素（MCC），采用了一种环保且可持续的方法。为了将酸浓度降至最低，使用 Taguchi 正交 L 设计优化了酸水解条件，该设计评估了反应时间、温度、酸浓度和固液比对 MCC 物理化学特性的影响。纤维素源在其不同的加工阶段都经过了各种分析技术的形态和物理化学研究。优化后的 MCC 产率最高可达 83%。由于使用了非常低（5% HSO）的酸浓度、低腐蚀性、减少了废水排放和降低了成本，因此该工艺是有利的。详细分析表明，分离出的 MCC 具有良好的结晶度和热稳定性，因此有望成为用于生产各种应用的聚合物生物复合材料的高价值前体。
