Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China; School of Bioengineering, East China University of Science and Technology, Shanghai 200237, China.
Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
J Proteomics. 2020 May 15;219:103737. doi: 10.1016/j.jprot.2020.103737. Epub 2020 Mar 18.
The gut microbiota is the largest and most complex microbial community in the human body. Host-gut microbiota interactions have significant implications on health and disease. The development of genome-sequencing technologies, especially the application of next-generation sequencing (NGS), has accelerated the study of the gut microbiota. Most gut microbiota studies rely on 16S rRNA sequencing, metagenomics, and metatranscriptomics, but metaproteomics, based on mass spectrometry (MS), provides functional information on the signaling and metabolic pathways in the gut microbiota. This review is intended to introduce different research methods to study the gut microbiota, with a specific focus on the current progress and application of metaproteomics. SIGNIFICANCE: The gut microbiota plays a key role in human health and disease. In this review, different research methods are described and compared in the field of the gut microbiota. Among these research methods, metaproteomics reveals the taxonomy and functionality of the gut microbiota, especially the functional pathways associated with diseases. Thus, the current progress and application of metaproteomics are summarized, in order to enhance a comprehensive depiction of metaproteomics.
肠道微生物群是人体中最大和最复杂的微生物群落。宿主-肠道微生物群相互作用对健康和疾病有重大影响。基因组测序技术的发展，特别是下一代测序（NGS）的应用，加速了对肠道微生物群的研究。大多数肠道微生物群研究依赖于 16S rRNA 测序、宏基因组学和宏转录组学，但基于质谱（MS）的代谢组学为肠道微生物群中的信号和代谢途径提供了功能信息。这篇综述旨在介绍研究肠道微生物群的不同方法，特别关注代谢组学的当前进展和应用。意义：肠道微生物群在人类健康和疾病中起着关键作用。在这篇综述中，描述并比较了肠道微生物群领域的不同研究方法。在这些研究方法中，代谢组学揭示了肠道微生物群的分类和功能，特别是与疾病相关的功能途径。因此，总结了代谢组学的当前进展和应用，以增强对代谢组学的全面描述。
