Faculty of Education, Secondary School Science and Mathematics Education, Biology Education, Gazi University, Ankara, Turkey.
Kaman High School of Applied Science, Kırşehir Ahi Evran University, Kırşehir, Turkey.
Environ Monit Assess. 2020 Mar 20;192(4):244. doi: 10.1007/s10661-020-8207-4.
The information available on microalgae-sourced compounds, especially antibiotics and other bioactive compounds, and their potential commercial applications is still insufficient. In this study, antibacterial activity, metabolites, and molecular characterization of Phormidium autumnale, which was isolated from samples collected from different natural freshwater sources in Ankara, Turkey, were investigated. Sequencing results of 16s rDNA confirmed the molecular identification of P. autumnale by 99%. It was determined that the peak values of some phenolic compounds and cyclic peptides were consistent with the 1653-1389 cm band regions in the FTIR spectra of the species. The antibacterial activities of P. autumnale cyanobacteria (CBA) extracts that were obtained by using different solvents were tested on Escherichia coli, Staphylococcus epidermidis, methicillin-resistant (MR) Staphylococcus aureus, Streptococcus agalactiae, and Enterococcus faecalis by using a disc diffusion method. Also, the minimum inhibition concentration (MIC) and antimicrobial indexes of all extracts were determined. It was found that P. autumnale methanol extracts showed antibacterial activity on all test bacteria, whereas acetone extracts showed effects only on E. coli. For the inhibition of MR S. aureus, the control methanol extract was found to give very similar results to those exhibited by the control antibiotics, and the antimicrobial index results were determined to be 58.7-67.5%. According to the results of the analysis of methanol extract, gentisic acid, vanillic acid, 4-hydroxybenzoic acid, p-coumaric acid, and catechin (especially phenolic compounds) were determined to be the active compounds. It can be concluded that P. autumnale is an alternative to current commercial applications as an antibacterial agent in phytotherapy.
从可用的信息来看，关于微藻来源的化合物，尤其是抗生素和其他生物活性化合物，及其潜在的商业应用，仍然不足。在这项研究中，研究了从土耳其安卡拉不同天然淡水来源采集的样本中分离出的 Phormidium autumnale 的抗菌活性、代谢产物和分子特征。16s rDNA 的测序结果通过 99%确认了 P. autumnale 的分子鉴定。确定了一些酚类化合物和环状肽的峰值与该物种的 FTIR 光谱的 1653-1389 cm 波段区域一致。采用不同溶剂获得的 P. autumnale 蓝藻 (CBA) 提取物的抗菌活性通过圆盘扩散法在大肠杆菌、表皮葡萄球菌、耐甲氧西林金黄色葡萄球菌、无乳链球菌和粪肠球菌上进行了测试。同时，还测定了所有提取物的最小抑菌浓度 (MIC) 和抗菌指数。结果表明，甲醇提取物对所有测试菌均具有抗菌活性，而丙酮提取物仅对大肠杆菌有作用。对于 MRSA 的抑制，发现对照甲醇提取物的结果与对照抗生素非常相似，抗菌指数结果为 58.7-67.5%。根据甲醇提取物的分析结果，确定龙胆酸、香草酸、对羟基苯甲酸、对香豆酸和儿茶素（特别是酚类化合物）为活性化合物。可以得出结论，P. autumnale 可作为植物疗法中的抗菌剂替代当前的商业应用。
