Brandon D M, Nayak S K, Binder P S
Ophthalmology Research Laboratory, Sharp Cabrillo Hospital, San Diego, California.
Cornea. 1988;7(4):257-66.
Twelve FITC-conjugated lectins were used to establish a reaction pattern with cellular and noncellular components in fresh-frozen and formalin-fixed paraffin sections of 11 normal human corneas. In frozen sections the epithelium demonstrated the most active lectin staining; keratocytes and endothelium stained to a lesser degree. Of the noncellular components, the epithelial basement membrane and Descemet's membrane of some of the tissues were stained most by Phasolus vulgaris agglutinin (PHA), Pisum sativum agglutinin (PSA), Concanavalin A (Con-A), and Sopora japonica agglutinin (SJA). None of the lectins stained Bowman's layer or the stromal matrix. Keratocytes in paraffin sections stained the most compared with the epithelium and endothelium. In paraffin sections, the epithelial basement membrane and Bowman's layer of some of the tissue sections were stained by Con-A, Ricinus cummuni agglutinin 120 (RCA-120), Banderiraea simplicifolia lectin (BSL-I), PHA, and wheat germ agglutinin (WGA). From this study, it was observed that the reactivity of lectins to the cellular components in paraffin-embedded sections was less pronounced than in frozen sections. Sensitivity of lectin binding was considerably enhanced by the proteolytic enzyme digestion of paraffin sections.
使用12种异硫氰酸荧光素（FITC）偶联的凝集素，在11例正常人角膜的新鲜冰冻切片和福尔马林固定石蜡切片中，与细胞和非细胞成分建立反应模式。在冰冻切片中，上皮细胞呈现出最活跃的凝集素染色；角膜细胞和内皮细胞染色程度较轻。在非细胞成分中，某些组织的上皮基底膜和Descemet膜被菜豆凝集素（PHA）、豌豆凝集素（PSA）、刀豆球蛋白A（Con-A）和皂荚凝集素（SJA）染色最深。没有一种凝集素能使Bowman层或基质染色。石蜡切片中的角膜细胞与上皮细胞和内皮细胞相比染色最深。在石蜡切片中，某些组织切片的上皮基底膜和Bowman层被Con-A、蓖麻凝集素120（RCA-120）、单叶豆凝集素（BSL-I）、PHA和小麦胚芽凝集素（WGA）染色。从这项研究中观察到，凝集素对石蜡包埋切片中细胞成分的反应性不如在冰冻切片中明显。通过对石蜡切片进行蛋白酶消化，凝集素结合的敏感性得到了显著提高。
