Kinscherf T G, Leong S A
Department of Plant Pathology, USDA-ARS, University of Wisconsin, Madison 53706.
Chromosoma. 1988;96(6):427-33. doi: 10.1007/BF00303036.
A molecular karyotype of the corn smut-inducing fungus Ustilago maydis was prepared using orthogonalfield-alternation gel electrophoresis (OFAGE). At least 20 chromosome-sized DNAs ranging from approximately 300 kb to the maximum limit of resolution of this system were identified in haploid cells of a widely used strain. Although general features of the banding pattern of chromosome-sized DNAs were conserved between strains, no two strains had identical karyotypes, indicating that considerable chromosome length polymorphism exists in this species. This polymorphism was seen in both laboratory strains as well as more recent isolates from nature. Length variation in apparently identical chromosomes was usually small, but was occasionally significant. In one strain Southern DNA hybridization analysis suggested the occurrence of a stable large-scale, inter-chromosomal exchange which had given rise to two novel chromosomes.
利用正交交变电场凝胶电泳（OFAGE）制备了玉米黑粉菌诱导真菌玉米黑粉菌的分子核型。在一个广泛使用的菌株的单倍体细胞中，鉴定出至少20条染色体大小的DNA，范围从大约300 kb到该系统分辨率的最大极限。尽管染色体大小的DNA条带模式的一般特征在菌株之间是保守的，但没有两个菌株具有相同的核型，这表明该物种中存在相当大的染色体长度多态性。这种多态性在实验室菌株以及最近从自然界分离的菌株中都可以看到。明显相同的染色体长度变化通常很小，但偶尔也很显著。在一个菌株中，Southern DNA杂交分析表明发生了稳定大规模的染色体间交换，从而产生了两条新染色体。
