Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
Chemosphere. 2020 Aug;252:126459. doi: 10.1016/j.chemosphere.2020.126459. Epub 2020 Mar 12.
A 98-day research was conducted to assess the influence of dietary montmorillonite (MMT) on growth and immune competence in rainbow trout (Oncorhynchus mykiss). Fish fry (mean weight: 500 ± 35 mg) were fed with four experimental diets contained MMT at levels of 0 (as control group), 1, 2 and 4%. At the end of the experiment, the survival rate and growth parameters and serum total protein and albumin levels in MMT experimental groups was higher than the control group. Regarding humoral immune responses, fish being fed with 2 and 4% MMT diets showed the greatest and the lowest serum C, C and lysozyme levels, respectively. Serum alternative complement pathway activity (ACH50) in groups fed with 1 and 2% MMT supplemented diets was higher than the other groups. At the end of the challenge test with VHSV, the mortality rate reached to 28 and 20% in the control group and 4% MMT supplemented groups, respectively, whereas fish fed with 1 and 2% MMT supplemented diets showed the lowest mortality rate (2.5%) Fish fed with 1 and 2% MMT supplemented groups showed higher head kidney IL-1β and TNF-α genes expression compared to the other treatment groups. In addition, fish fed with 2% MMT-supplemented diet had the highest head kidney Lyz-2 gene expression. In summary, the results of this research suggests that supplementing diet with MMT at 1 or 2% improve growth and non-specific humoral immune responses and induce head kidney immune relate genes expression and disease resistance against VHS in O. mykiss.
进行了为期 98 天的研究，以评估日粮蒙脱石（MMT）对虹鳟（Oncorhynchus mykiss）生长和免疫能力的影响。鱼苗（平均体重：500±35mg）用四种含有 MMT 的实验饲料喂养，MMT 水平分别为 0（作为对照组）、1、2 和 4%。实验结束时，MMT 实验组的存活率和生长参数以及血清总蛋白和白蛋白水平均高于对照组。在体液免疫反应方面，饲喂 2%和 4%MMT 日粮的鱼分别表现出最高和最低的血清 C3、C4 和溶菌酶水平。饲喂 1%和 2%MMT 补充日粮的鱼的血清替代补体途径活性（ACH50）高于其他组。在 VHSV 攻毒试验结束时，对照组和 4%MMT 补充组的死亡率分别达到 28%和 20%，而饲喂 1%和 2%MMT 补充日粮的鱼死亡率最低（2.5%）。饲喂 1%和 2%MMT 补充日粮的鱼头肾 IL-1β和 TNF-α基因表达高于其他处理组。此外，饲喂 2%MMT 补充日粮的鱼头肾 Lyz-2 基因表达最高。总之，这项研究的结果表明，日粮中添加 1%或 2%的 MMT 可提高虹鳟的生长和非特异性体液免疫反应，并诱导头肾免疫相关基因表达，提高虹鳟对 VHS 的抗病能力。
