Biophysics Department, Faculty of Science, Cairo University, Giza, Egypt.
Naunyn Schmiedebergs Arch Pharmacol. 2020 Aug;393(8):1475-1485. doi: 10.1007/s00210-020-01855-9. Epub 2020 Mar 21.
The present work was to examine a combination of therapy for a low dose of cisplatin and a magnetic field (MF) on Ehrlich carcinoma-bearing mice. In this study, a total of 50 BALB/C female mice were equally distributed into five groups. Mice from the control group did not receive MF or cisplatin. The low and high dose cisplatin groups were injected intraperitoneal (i.p.) with 3 and 6 mg/kg cisplatin, respectively, on the experimental days (1, 4, and 8). Mice group of cisplatin + MF was injected with a low dose of cisplatin followed by MF exposure (50 Hz, 50 mT), and the MF group was exposed to MF only. The impact of MF and cisplatin on the tumor and kidney were evaluated by measuring superoxide dismutase (SOD) activity, malondialdehyde (MDA) and glutathione (GSH) levels, DNA injury (comet assay), histopathological investigation of tissues, and tumor progress. The results suggested that the combination of a low dose of cisplatin with MF was significantly elevated in MDA levels, reduced SOD activity, and GSH levels. Furthermore, it caused a rise in comet parameters and inhibition in tumor growth. These results showed that MF enhances the therapeutic efficacy of low cisplatin doses and reduces nephrotoxicity.
本研究旨在探讨低剂量顺铂联合磁场（MF）对艾氏腹水癌荷瘤小鼠的治疗作用。本研究共使用 50 只 BALB/C 雌性小鼠，平均分为 5 组。对照组小鼠不接受 MF 或顺铂处理。低剂量和顺铂高剂量组分别在实验第 1、4、8 天腹腔注射 3 和 6mg/kg 的顺铂。顺铂+MF 组先给予低剂量顺铂，然后进行 MF 暴露（50Hz，50mT），MF 组仅进行 MF 暴露。通过测量超氧化物歧化酶（SOD）活性、丙二醛（MDA）和谷胱甘肽（GSH）水平、DNA 损伤（彗星试验）、组织病理学检查以及肿瘤进展来评估 MF 和顺铂对肿瘤和肾脏的影响。结果表明，低剂量顺铂联合 MF 处理后 MDA 水平显著升高，SOD 活性和 GSH 水平降低。此外，MF 还导致彗星参数升高和肿瘤生长抑制。这些结果表明，MF 增强了低剂量顺铂的治疗效果，同时降低了顺铂的肾毒性。
