Biophysics Department, Faculty of Science, Cairo University, Giza, Egypt.
Naunyn Schmiedebergs Arch Pharmacol. 2018 Dec;391(12):1421-1430. doi: 10.1007/s00210-018-1558-5. Epub 2018 Sep 3.
Silver nanoparticles (AgNPs) have a wide range of industrial and biomedical applications. The aim of the present study was to determine the cytotoxic and genotoxic effects of AgNPs on Ehrlich carcinoma-bearing mice. AgNPs were characterized by ultraviolet-visible absorption spectroscopy, dynamic light scattering, and transmission electron microscopy (TEM). Furthermore, the cytotoxicity and genotoxicity of AgNPs were evaluated using a series of assays: superoxide dismutase (SOD) enzyme activity, malondialdehyde (MDA) levels, DNA damage (comet assay), and histopathological examination of tissues and tumor size in Ehrlich carcinoma-bearing mice. Treatment of Ehrlich carcinoma-bearing mice with various concentrations of AgNPs (6, 24, and 48 mg/kg) injected intra peritoneal (IP) and intra tumor (IT) revealed that AgNPs significantly elevated the levels (0.5- to 5-fold) of MDA and reduced the activity (32-64%) of SOD. Furthermore, AgNPs caused a 2- to 3-fold increase in comet parameters such as percent tail DNA. Additionally, AgNPs inhibit the promotion of Ehrlich carcinoma by masses of necrotic and fragmented tumor cells. Consequently, the volume of tumor reduced by about 31-95% compared to control one. The results indicate that AgNPs possess cytotoxic and genotoxic effects against Ehrlich tumor and confirm the antitumor properties of AgNPs.
银纳米粒子（AgNPs）在工业和生物医学领域有广泛的应用。本研究旨在确定 AgNPs 对艾氏腹水癌荷瘤小鼠的细胞毒性和遗传毒性作用。采用紫外-可见吸收光谱、动态光散射和透射电子显微镜（TEM）对 AgNPs 进行了表征。此外，还通过一系列检测方法评估了 AgNPs 的细胞毒性和遗传毒性：超氧化物歧化酶（SOD）酶活性、丙二醛（MDA）水平、DNA 损伤（彗星试验）以及艾氏腹水癌荷瘤小鼠组织的组织病理学检查和肿瘤大小。用不同浓度的 AgNPs（6、24 和 48 mg/kg）腹腔内（IP）和肿瘤内（IT）注射艾氏腹水癌荷瘤小鼠，结果表明 AgNPs 显著提高了 MDA 的水平（0.5-5 倍），降低了 SOD 的活性（32-64%）。此外，AgNPs 使彗星参数如尾 DNA 的百分比增加了 2-3 倍。此外，AgNPs 抑制了艾氏腹水癌细胞的增殖，大量坏死和破碎的肿瘤细胞被抑制。因此，与对照组相比，肿瘤体积减少了约 31-95%。结果表明，AgNPs 对艾氏腹水瘤具有细胞毒性和遗传毒性作用，证实了 AgNPs 的抗肿瘤特性。
