Murao Masanobu, Imano Tetsuo, Akiyama Junichi, Kawakami Teruhiko, Nakajima Masaaki
Graduate School of Health Science, Kibi International University, Takahashi, Japan.
Department of Physical Therapy, Fukuyama Medical College, Fukuyama, Japan.
Growth Factors. 2019 Dec;37(5-6):257-262. doi: 10.1080/08977194.2020.1742118. Epub 2020 Mar 21.
This study aimed to characterize the effect of different running modes on serum irisin concentrations in rats. A total of 18, 10-week-old rats were divided into three groups; control group, 16° uphill running group (concentric exercise; CON) and, -16° downhill running group (eccentric exercise; ECC). The running group's rats ran on the inclined treadmill at 16 m/min, for a total of 90 min. Blood was drawn from the rats, 48 h after running, after which the rats were anesthetized. The serum concentrations of irisin were measured using enzyme-linked immunosorbent assays. Vastus intermedius was collected for immunohistochemical analysis. After multiple comparisons, the ECC showed a significantly high serum irisin concentration (ECC: 28.42 ± 6.31 ng/ml, CON: 21.27 ± 3.03 ng/ml) and a larger irisin antibody reactive cross-sectional area in vastus intermedius compared to the CON ( < 0.05). This is the first study to reveal that single bout downhill running increases serum irisin concentrations in rats.
