Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing 10080, China.
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China.
J Chromatogr A. 2020 Jun 21;1621:461031. doi: 10.1016/j.chroma.2020.461031. Epub 2020 Mar 19.
An innovative strong hydrophilic organic polymer monolithic column of poly(N-benzylquininium chloride-co-1, 3, 5-triacryloylhexahydro-1, 3, 5-triazine) (poly(NBQ-co-TAT)) has been successfully synthesized through in situ copolymerization for capillary electrochromatography. The amphiphilic monomer NBQ and the strong polar cross-linker TAT are firstly used in hydrophilic electrochromatography by taking advantage of the exhibition of hydrophilicity at lower levels of organic solvent and ease formation of porous structure. The monolithic column poly(NBQ-co-TAT) shows powerful hydrophilic selectivity with mobile phase containing more than 60% organic solvent. The introduction of NBQ and TAT enlarges the sources of functional monomers and cross-linkers for HILIC. Due to the presence of the positively charged group in NBQ, an anodic electroosmotic flow is generated with the change of pH values from 2.0 to 12.0. The monolithic column was used for the separations of thioureas, phenols, xanthines, nucleobases, acidic substances and pharmaceuticals. The highest column efficiency for N, N'-dimethylthiourea is 1.15 × 10 N m. The application of the monolithic column for a real sample, cytochrome C digestion indicates its great potential in practical application.
通过原位共聚成功合成了一种用于毛细管电色谱的新型强亲水性有机聚合物整体柱聚（N-苄基氯化喹宁-co-1,3,5-三丙烯酰基六氢-1,3,5-三嗪）（poly（NBQ-co-TAT））。利用低有机溶剂水平下表现出的亲水性和易于形成多孔结构的优势，首次将双亲水性单体 NBQ 和强极性交联剂 TAT 用于亲水电色谱中。整体柱 poly（NBQ-co-TAT）在含有超过 60%有机溶剂的流动相中表现出强大的亲水选择性。NBQ 和 TAT 的引入扩大了 HILIC 中功能单体和交联剂的来源。由于 NBQ 中存在正电荷基团，在 pH 值从 2.0 到 12.0 的变化下会产生阳极电渗流。该整体柱用于硫脲、酚类、黄嘌呤、碱基、酸性物质和药物的分离。对 N,N'-二甲基硫脲的最高柱效为 1.15×10 N m。该整体柱在实际样品细胞色素 C 消化中的应用表明了其在实际应用中的巨大潜力。
