刺突蛋白对哺乳动物 ACE2 的识别预测了宿主范围和用于 SARS-CoV-2 感染的优化 ACE2。
Spike protein recognition of mammalian ACE2 predicts the host range and an optimized ACE2 for SARS-CoV-2 infection.
机构信息
Institute of Basic Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250062, Shandong, China.
Institute of Basic Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250062, Shandong, China; School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan, 250200, Shandong, China.
出版信息
Biochem Biophys Res Commun. 2020 May 21;526(1):165-169. doi: 10.1016/j.bbrc.2020.03.047. Epub 2020 Mar 19.
SARS-CoV-2 causes the recent global COVID-19 public health emergency. ACE2 is the receptor for both SARS-CoV-2 and SARS-CoV. To predict the potential host range of SARS-CoV-2, we analyzed the key residues of ACE2 for recognizing S protein. We found that most of the selected mammals including pets (dog and cat), pangolin and Circetidae mammals remained the most of key residues for association with S protein from SARS-CoV and SARS-CoV-2. The interaction interface between cat/dog/pangolin/Chinese hamster ACE2 and SARS-CoV/SARS-CoV-2 S protein was simulated through homology modeling. We identified that N82 in ACE2 showed a closer contact with SARS-CoV-2 S protein than M82 in human ACE2. Our finding will provide important insights into the host range of SARS-CoV-2 and a new strategy to design an optimized ACE2 for SARS-CoV-2 infection.
SARS-CoV-2 引发了最近的全球 COVID-19 公共卫生紧急事件。ACE2 是 SARS-CoV-2 和 SARS-CoV 的受体。为了预测 SARS-CoV-2 的潜在宿主范围，我们分析了 ACE2 识别 S 蛋白的关键残基。我们发现，包括宠物（狗和猫）、穿山甲和 Circetidae 哺乳动物在内的大多数选定哺乳动物仍然保留了与 SARS-CoV 和 SARS-CoV-2 的 S 蛋白结合的大多数关键残基。通过同源建模模拟了猫/狗/穿山甲/中国仓鼠 ACE2 与 SARS-CoV/SARS-CoV-2 S 蛋白之间的相互作用界面。我们发现 ACE2 中的 N82 与 SARS-CoV-2 S 蛋白的接触比人类 ACE2 中的 M82 更紧密。我们的发现将为 SARS-CoV-2 的宿主范围提供重要的见解，并为设计针对 SARS-CoV-2 感染的优化 ACE2 提供新策略。
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