Panayotis N. Soucacos", Orthopaedic Research & Education Center (OREC), 1 Rimini Street, Attikon University Hospital, Haidari 124 62 Athens, Greece.
Panayotis N. Soucacos", Orthopaedic Research & Education Center (OREC), 1 Rimini Street, Attikon University Hospital, Haidari 124 62 Athens, Greece.
Injury. 2020 Dec;51(12):2855-2865. doi: 10.1016/j.injury.2020.03.022. Epub 2020 Mar 9.
Engineering complex tissues is perhaps the most ambitious goal of all tissue engineers. Despite significant advances in tissue engineering, which have resulted in successful engineering of simple tissues such as skin and cartilage, there are a number of challenges that remain in engineering of complex, hybrid tissue structures, such as osteochondral tissue. Mesenchymal stem cells (MSCs) have the capacity to highly proliferate in an undifferentiated state and the potential to differentiate into a variety of different lineages, providing a promising single cell source to produce multiple cell types. MSC obtained from adult human contribute to the regeneration of mesenchymal tissues such as bone, cartilage, fat, muscle, tendon and marrow stroma. In the present study, the regeneration capacity of multipotent MSCs derived from different tissues in the rabbit were compared. Specifically the aim of this study was to isolate and characterize rabbit adult stem cell populations from bone marrow, adipose, synovial membrane, rotator cuff, ligament and tendon and assess their cell morphology, growth rate, cell surface markers and differentiation capacity. MSCs derived from synovial membrane showed superiority in terms of chondrogenesis, osteogenesis, myogenesis and tenogenesis, suggesting that synovial membrane-derived MSCs would be a good candidate for efforts to regenerate musculoskeletal tissues.
工程复杂组织可能是所有组织工程师的最宏伟目标。尽管组织工程学取得了重大进展，成功地工程化了简单组织如皮肤和软骨，但在工程复杂、混合组织结构方面，如骨软骨组织，仍存在许多挑战。间充质干细胞（MSCs）具有在未分化状态下高度增殖的能力，并且有可能分化为多种不同的谱系，为产生多种细胞类型提供了有前途的单一细胞来源。从成人获得的 MSC 有助于间充质组织如骨、软骨、脂肪、肌肉、肌腱和骨髓基质的再生。在本研究中，比较了来自兔不同组织的多能 MSC 的再生能力。具体而言，本研究的目的是从骨髓、脂肪、滑膜、肩袖、韧带和肌腱中分离和鉴定兔成体干细胞群体，并评估它们的细胞形态、生长速度、细胞表面标志物和分化能力。滑膜来源的 MSC 在软骨形成、成骨、成肌和成腱方面表现出优势，这表明滑膜来源的 MSC 将是再生肌肉骨骼组织的良好候选者。
