Zhou Fang, Feng Huan, Li Haibo, Wang Yue, Zhang Zhiqiang, Kang Wenjun, Jia Hongmin, Yang Xinyi, Meng Qingtao, Zhang Run
School of Chemical Engineering, University of Science and Technology Liaoning, Anshan, Liaoning 114051, China.
Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, Department of Chemistry, Liaocheng University, Liaocheng 252059, China.
ACS Omega. 2020 Mar 3;5(10):5452-5459. doi: 10.1021/acsomega.0c00063. eCollection 2020 Mar 17.
Key roles of bisulfite (HSO ) in food quality assurance and human health necessitate a reliable analytical method for rapid, sensitive, and selective detection of HSO . Herein, a new red-emitting ratiometric fluorescence probe, , is reported for sensitive and selective detection of HSO in food samples and live animals. Probe recognizes HSO via a 1,4-nucleophilic addition reaction. As a result of this specific reaction, emission intensities at 625 and 475 nm are dramatically changed, allowing the detection of HSO in a ratiometric fluorescence model in an aqueous solution. The obvious changes of solution color from pink to transparent and fluorescence color from rose-red to cyan allow the detection of HSO by naked eyes. Furthermore, probe has fast response in color and fluorescence (<2 min), excellent selectivity, and a low detection limit (0.29 μM), which enables its application in HSO detection in food samples and live organisms. The practical applications of probe are then demonstrated by the visualization of HSO in live animals (zebrafish and nude mouse) as well as the determination of HSO in white wine and sugar.
亚硫酸氢根（HSO₃⁻）在食品质量保证和人类健康方面的关键作用使得需要一种可靠的分析方法来快速、灵敏且选择性地检测HSO₃⁻。在此，报道了一种新型的发红光比率荧光探针，用于在食品样品和活体动物中灵敏且选择性地检测HSO₃⁻。探针通过1,4-亲核加成反应识别HSO₃⁻。由于这种特定反应，625和475nm处的发射强度发生显著变化，从而能够在水溶液中的比率荧光模型中检测HSO₃⁻。溶液颜色从粉红色变为透明以及荧光颜色从玫瑰红色变为青色的明显变化使得能够通过肉眼检测HSO₃⁻。此外，探针在颜色和荧光方面具有快速响应（<2分钟）、优异的选择性和低检测限（0.29μM），这使其能够应用于食品样品和活生物体中HSO₃⁻的检测。然后通过在活体动物（斑马鱼和裸鼠）中可视化HSO₃⁻以及测定白葡萄酒和糖中的HSO₃⁻来证明探针的实际应用。
