Department of Pulmonary Medicine, Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
Internal Medicine, Qingdao Municipal Hospital, Qingdao, Shandong, China.
Integr Cancer Ther. 2020 Jan-Dec;19:1534735420911430. doi: 10.1177/1534735420911430.
Sea cucumber is a kind of food. Holothurian glycosaminoglycan (hGAG) is extracted from the body wall of the sea cucumber. Administration of hGAG and cisplatin (DDP) together to treat lung cancer was investigated. Lung adenocarcinoma A549 cells were cultured and divided into 4 groups: control group, hGAG 100 µg/mL group, DDP 3 µg/mL group, and hGAG 100 µg/mL + DDP 3 µg/mL group. Cell inhibition and apoptosis was evaluated by CCK8 and Hoechst33258 staining. Cell cycle was tested by Annexin V-FITC/PI (propidium iodide) double-staining and flow cytometry. The expression of mRNA and protein of Bcl-2, Bax, caspase-3, and survivin were detected by reverse transcriptase-polymerase chain reaction and Western blot, respectively. The results showed that hGAG combined with DDP enhanced the inhibitory effect of DDP on A549 lung cells through apoptosis pathway. The mechanism of apoptosis may be related to the reduction of Bcl-2 and survivin, as well as the ascension of Bax and caspase-3. hGAG could promote A549 cell cycle arrest in G1 and G2 phase and improve the DDP chemotherapy effects on A549 cells.
海参是一种食物。海参糖胺聚糖（hGAG）是从海参体壁中提取的。研究了 hGAG 与顺铂（DDP）联合治疗肺癌的效果。培养肺腺癌 A549 细胞，并将其分为 4 组：对照组、hGAG100μg/mL 组、DDP3μg/mL 组和 hGAG100μg/mL+DDP3μg/mL 组。通过 CCK8 和 Hoechst33258 染色评估细胞抑制和凋亡情况。通过 Annexin V-FITC/PI（碘化丙啶）双染和流式细胞术检测细胞周期。通过逆转录-聚合酶链反应和 Western blot 分别检测 Bcl-2、Bax、caspase-3 和 survivin 的 mRNA 和蛋白表达。结果表明，hGAG 联合 DDP 通过凋亡途径增强了 DDP 对 A549 肺细胞的抑制作用。凋亡的机制可能与 Bcl-2 和 survivin 的减少以及 Bax 和 caspase-3 的上升有关。hGAG 可促进 A549 细胞周期停滞在 G1 和 G2 期，并提高 DDP 对 A549 细胞的化疗效果。
