Hatta H, Atomi Y, Shinohara S, Yamamoto Y, Yamada S
Laboratory for Exercise Physiology and Biomechanics, Faculty of Education, University of Tokyo, Japan.
Horm Metab Res. 1988 Oct;20(10):609-11. doi: 10.1055/s-2007-1010897.
The effects of ovarian hormones on glucose and fatty acid oxidation during exercise were investigated in adult female ovariectomized rats. Rats subdivided into 3 groups received intraperitoneal injections of hormones or sesame oil for 8 days. Estrogen (E) treated rats received 17-beta estradiol in daily doses of 2 micrograms. Estrogen and progesterone treated rats (EP) received 17-beta estradiol in daily doses of 2 micrograms and 2 mg, respectively. Control rats (S) received sesame oil alone. After an overnight fast, rats ran at the speed of 25 m.min-1 for 60 min. [U-14C]glucose or [1-14C]palmitate was injected into rats at 5 min of exercise and before 10 min of exercise, respectively. Expired 14CO2 was collected using bottomless chamber on a treadmill belt. No significant differences were found in mean blood glucose, lactate and plasma free fatty acid concentrations after the exercise. Until the end of the exercise 34.7 +/- 2.6 (E, n = 5), 40.8 +/- 2.9 (EP, n = 5) and 43.7 +/- 3.5% (S, n = 6) (mean +/- SE) of 14C which was injected as 14C-glucose was recovered as 14CO2. During 60 min of the exercise 27.5 +/- 1.0 (E, n = 7), 19.8 +/- 2.7 (EP, n = 6) and 25.0 +/- 1.9% (S, n = 6) of 14C which was injected as 14C-palmitate was recovered as 14CO2. A significant difference was found in this rate between E and EP (P less than 0.05). It was concluded that estrogen treatment stimulated fatty acid oxidation compared with the estrogen plus progesterone treatment and tended to inhibit glucose oxidation during prolonged exercise.
在成年雌性去卵巢大鼠中研究了卵巢激素对运动期间葡萄糖和脂肪酸氧化的影响。将大鼠分为3组，腹腔注射激素或芝麻油，持续8天。雌激素（E）处理组大鼠每日接受2微克的17-β-雌二醇。雌激素和孕酮处理组（EP）大鼠分别每日接受2微克和2毫克的17-β-雌二醇。对照组大鼠（S）仅接受芝麻油。禁食过夜后，大鼠以25米/分钟的速度奔跑60分钟。在运动5分钟和运动前10分钟分别向大鼠注射[U-14C]葡萄糖或[1-14C]棕榈酸酯。使用跑步机皮带上的无底箱收集呼出的14CO2。运动后平均血糖、乳酸和血浆游离脂肪酸浓度未发现显著差异。运动结束时，作为14C-葡萄糖注射的14C中有34.7±2.6（E组，n = 5）、40.8±2.9（EP组，n = 5）和43.7±3.5%（S组，n = 6）（平均值±标准误）以14CO2的形式回收。在60分钟的运动期间，作为14C-棕榈酸酯注射的14C中有27.5±1.0（E组，n = 7）、19.8±2.7（EP组，n = 6）和25.0±1.9%（S组，n = 6）以14CO2的形式回收。E组和EP组在该速率上存在显著差异（P < 0.05）。得出的结论是，与雌激素加孕酮治疗相比，雌激素治疗在长时间运动期间刺激脂肪酸氧化，并倾向于抑制葡萄糖氧化。
