Laboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea.
Department of Biomedical Laboratory Science; and Molecular Diagnostics Research Institute, Namseoul University, Cheonan, 31020, Republic of Korea.
BMC Complement Med Ther. 2020 Mar 23;20(1):98. doi: 10.1186/s12906-020-02889-5.
The Rumex acetosa has been used in medicinal treatment, food technology and phytotherapeutics in Eastern Asia and many other countries. However, its effect on cardiovascular system and antiplatelet activity remained to be known. In this study, we examined the antiplatelet activity of R. acetosa in detailed manner to understand underlying mechanism.
To study this, whole blood was obtained from male Sprague Dawley (SD) rats and aggregation of washed platelets measured using light transmission aggregometry. Intracellular calcium ion concentration ([Ca]) was measured using Fura-2/AM while ATP release evaluated by luminometer. Activation of integrin αβ analyzed by flow cytometry and clot retraction. Furthermore, we studied the signaling pathways mediated by R. acetosa extract by western blot analysis.
R. acetosa extract markedly inhibited collagen-induced platelet aggregation and ATP release in a dose-dependent manner. It also suppressed [Ca] mobilization, integrin αβ activation and clot retraction. The extract significantly attenuated phosphorylation of the MAPK pathway (i.e., ERK1/2, JNK), MKK4, PI3K/Akt, and Src family kinase.
Taken together, this data suggests that R. acetosa extract exhibits anti-platelet activity via modulating MAPK, PI3K/Akt pathways, and integrin αβ-mediated inside-out and outside-in signaling, and it may protect against the development of platelet-related cardiovascular diseases.
酸模在东亚和许多其他国家被用于医学治疗、食品技术和植物疗法。然而，其对心血管系统和抗血小板活性的影响仍有待了解。在这项研究中，我们详细研究了酸模的抗血小板活性，以了解其潜在机制。
为了研究这一点，从雄性 Sprague Dawley（SD）大鼠中获得全血，并使用透光比浊法测量洗涤血小板的聚集。使用 Fura-2/AM 测量细胞内钙离子浓度([Ca])，并通过发光计评估 ATP 释放。通过流式细胞术分析整合素 αβ 的激活和血凝块回缩。此外，我们通过 Western blot 分析研究了酸模提取物介导的信号通路。
酸模提取物明显抑制胶原诱导的血小板聚集和 ATP 释放，呈剂量依赖性。它还抑制 [Ca]动员、整合素 αβ 激活和血凝块回缩。该提取物显著减弱 MAPK 通路（即 ERK1/2、JNK）、MKK4、PI3K/Akt 和 Src 家族激酶的磷酸化。
综上所述，这些数据表明，酸模提取物通过调节 MAPK、PI3K/Akt 通路以及整合素 αβ 介导的内外信号转导发挥抗血小板作用，并且可能预防血小板相关心血管疾病的发生。
