Jeon Bo-Ra, Irfan Muhammad, Lee Seung Eun, Lee Jeong Hoon, Rhee Man Hee
Laboratory of Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science (NIHHS), Eumseong 27709, Republic of Korea.
Evid Based Complement Alternat Med. 2018 Aug 5;2018:3835021. doi: 10.1155/2018/3835021. eCollection 2018.
Platelets play major role in maintaining hemostasis while hyperactivation of platelets may lead to arterial thrombosis. Natural products and ethnomedicine have been shown to reduce the risk of cardiovascular diseases (CVDs). is a perennial herb found in China, Korea, Russia, and Japan, which is also known for its medicinal effects, and has been used in Korean traditional medicine to treat inflammation, cancer, chronic bronchitis, and headache. We hypothesized that given herbal plant exhibits pharmacological activities against CVDs, and we specifically explored their effects on platelet function.
Platelet aggregation was evaluated using standard light-transmission aggregometry. Intracellular calcium mobilization was assessed using Fura-2/AM, and granule secretion (ATP release) was measured in a luminometer. Fibrinogen binding to integrin was assessed using flow cytometry. Phosphorylation of mitogen-activated protein kinase (MAPK) signaling molecules and activation of the phosphoinositide 3-kinase (PI3K)/Akt were assessed using western blots, and further, glycoprotein VI (GPVI) signaling components were studied using immunoprecipitation.
extracts potently and significantly inhibited platelet aggregation, calcium mobilization, granule secretion, and fibrinogen binding to integrin . Moreover, it significantly inhibited MAPK phosphorylation and expression of GPVI downstream signaling molecules.
extract inhibited platelet aggregation and granule secretion and attenuated GPVI downstream signaling, indicating the potential therapeutic effects of this plant extract on the cardiovascular system and platelet function. We suggest that given plant extract may be a potent candidate to treat platelet-related CVDs and to be used as antiplatelet agent.
血小板在维持止血过程中起主要作用，而血小板的过度活化可能导致动脉血栓形成。天然产物和民族医学已被证明可降低心血管疾病（CVD）的风险。[植物名称]是一种在中国、韩国、俄罗斯和日本均有发现的多年生草本植物，它也因其药用功效而闻名，并已被用于韩国传统医学中治疗炎症、癌症、慢性支气管炎和头痛。我们假设这种草药植物具有针对心血管疾病的药理活性，并特别探讨了它们对血小板功能的影响。
使用标准光透射聚集法评估血小板聚集。使用Fura-2/AM评估细胞内钙动员，并在发光计中测量颗粒分泌（ATP释放）。使用流式细胞术评估纤维蛋白原与整合素[整合素名称]的结合。使用蛋白质免疫印迹法评估丝裂原活化蛋白激酶（MAPK）信号分子的磷酸化和磷酸肌醇3激酶（PI3K）/Akt的激活，此外，使用免疫沉淀法研究糖蛋白VI（GPVI）信号成分。
[植物名称]提取物有效且显著地抑制了血小板聚集、钙动员、颗粒分泌以及纤维蛋白原与整合素[整合素名称]的结合。此外，它还显著抑制了MAPK磷酸化和GPVI下游信号分子的表达。
[植物名称]提取物抑制了血小板聚集和颗粒分泌，并减弱了GPVI下游信号传导，表明这种植物提取物对心血管系统和血小板功能具有潜在的治疗作用。我们认为这种植物提取物可能是治疗与血小板相关的心血管疾病以及用作抗血小板药物的有力候选物。
