Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran; Central Iran Research Complex, Nuclear Science and Technology Research Institute, Yazd, Iran.
Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
Int J Biol Macromol. 2020 Jul 1;154:962-973. doi: 10.1016/j.ijbiomac.2020.03.165. Epub 2020 Mar 20.
Two hydrogels of O-carboxymethyl chitosan conjugated with caffeic acid and its composite with polyacrylamide were synthesized using electron beam irradiation. The synthesized hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, scanning electron microscopy, and mechanical properties studies. The hydrogels were loaded with doxycycline by swelling and its release was investigated in various media. The effect of the dose of electron beam irradiation and PAAm amount on the properties of hydrogels including swelling, drug loading, drug release, mechanical properties, and gel content were studied. The release of doxycycline form hydrogels in different media obeyed the mechanism of non-Fickian diffusion and best fitted to the Higuchi model and Korsmeyer-Peppas. In-vitro doxycycline release consideration indicated that the drug's release from composite hydrogel occurs with higher amounts than the other one. The cytotoxic study confirmed the non-toxicity of the prepared hydrogels dressing. Moreover, the growth inhibition of permissive bacteria against Staphylococcus aureus and Escherichia coli were observed for doxycycline-loaded hydrogels. So, the synthesized hydrogels are appropriate for practical application as a new antibacterial wound dressing.
两种水凝胶，一种是用电子束辐照合成的 O-羧甲基壳聚糖接枝咖啡酸，另一种是其与聚丙烯酰胺的复合物。用傅里叶变换红外光谱、X 射线衍射、热重分析、扫描电子显微镜和力学性能研究对合成的水凝胶进行了表征。水凝胶通过溶胀负载了强力霉素，并在不同介质中研究了其释放情况。研究了电子束辐照剂量和 PAAm 用量对水凝胶的溶胀、药物负载、药物释放、力学性能和凝胶含量等性能的影响。强力霉素从水凝胶在不同介质中的释放遵循非 Fickian 扩散机制，最符合 Higuchi 模型和 Korsmeyer-Peppas。体外强力霉素释放考虑表明，与另一种水凝胶相比，复合水凝胶中药物的释放量更高。细胞毒性研究证实了所制备的水凝胶敷料无毒性。此外，还观察到负载强力霉素的水凝胶对金黄色葡萄球菌和大肠杆菌等允许细菌的生长抑制作用。因此，所合成的水凝胶适合作为新型抗菌伤口敷料实际应用。
