Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 56212, Republic of Korea; Radiation Science and Technology, University of Science and Technology, Daejeon, 34113, Republic of Korea.
Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 56212, Republic of Korea.
Carbohydr Polym. 2021 Apr 15;258:117718. doi: 10.1016/j.carbpol.2021.117718. Epub 2021 Jan 30.
Herein, carboxymethyl chitosan and poly(vinylpyrrolidone) based hydrogels were synthesized by electron beam irradiation with dose variations (15 kGy, 30 kGy, and 45 kGy) for drug delivery applications. Irradiation crosslinked hydrogels were characterized for swellings in different medias, chemical, thermal, cell cytotoxicity, and drug release aspects. Swelling analysis was evaluated in distilled water, buffer, and saline solutions. Fourier transform infrared analysis revealed the establishment of physical interactions and confirmed the presence of functional groups present in the drug carriers. Scanning electron microscopy depicted the porous structure, which is responsible for swelling, drug loading, and release. Cell cytotoxicity assays indicated good cell viability on RAW 264.7 cells and anticancer activity on cancerous AGS cell lines. Cumulative drug release (%) of kanamycin in PBS at pH 7.4 was more than 90 % at 168 h. These drug carriers show promise to be developed as a sustained drug delivery system.
本文通过电子束辐照合成了基于羧甲基壳聚糖和聚乙烯吡咯烷酮的水凝胶，辐照剂量分别为 15 kGy、30 kGy 和 45 kGy，用于药物输送应用。辐照交联水凝胶的溶胀性能在不同介质中进行了表征，包括化学、热、细胞毒性和药物释放方面。溶胀分析在蒸馏水中、缓冲液和盐溶液中进行评估。傅里叶变换红外分析显示建立了物理相互作用，并证实了药物载体中存在的官能团。扫描电子显微镜描述了多孔结构，这是导致溶胀、药物负载和释放的原因。细胞毒性试验表明 RAW 264.7 细胞具有良好的细胞活力，对癌细胞 AGS 细胞系具有抗癌活性。在 pH 值为 7.4 的 PBS 中，卡那霉素的累积药物释放（%）在 168 小时后超过 90%。这些药物载体有望开发为一种可持续的药物输送系统。
