Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts; and.
Boston University School of Medicine, Boston, Massachusetts.
Dermatol Surg. 2020 Dec;46(12):e146-e153. doi: 10.1097/DSS.0000000000002381.
Microneedling- and laser-assisted drug delivery are emerging techniques used to treat various conditions. However, key parameters affecting drug penetration remain unknown.
This study aims to investigate the importance of timing of topical application, needle length, and device type for drug delivery.
Skin harvested from cosmetic surgeries was treated with black ink applied before or after treatment with a microneedling pen (MP), roller, or fractional ablative CO2 laser, and incubated for different time intervals. Ink penetration was additionally tested using different needle lengths. Sandwich estimator was used for statistical analysis.
Ink applied before MP penetrated deeper compared to ink applied afterward at 1 and 3 hours, and roller microneedling in both the ink-before and -after scenarios at 1, 3, and 6 hours (p < .05). Microneedling demonstrated lateral extension of ink beyond microchannels with increased ink penetration over time. CO2 laser demonstrated ink localization within microthermal zones without time-dependent increases in depth after 30 minutes. Ink penetration increases by 0.06 mm per 1 mm increase in needle length.
Ink applied before MP results in the deepest penetration of ink. Microneedling offers unique advantages in transdermal delivery as its channels exhibit increasing penetration over time and lateral extension of product.
微针和激光辅助药物输送是用于治疗各种疾病的新兴技术。然而，影响药物渗透的关键参数尚不清楚。
本研究旨在探讨局部应用时间、针长和设备类型对药物输送的重要性。
从美容手术中采集皮肤，在使用微针笔（MP）、滚轮或分数性 CO2 激光治疗之前或之后应用黑色墨水，并在不同的时间间隔下孵育。还使用不同的针长测试墨水的渗透。使用 sandwich 估计器进行统计分析。
与治疗后相比，MP 治疗前应用的墨水在 1 小时和 3 小时时渗透更深，在墨水治疗前和治疗后 1、3 和 6 小时时滚轮微针处理的墨水渗透更深（p <.05）。微针处理显示墨水在微通道之外的横向扩展，随着时间的推移，墨水渗透增加。CO2 激光显示墨水分区位于微热区，30 分钟后深度无时间依赖性增加。针长每增加 1 毫米，墨水渗透增加 0.06 毫米。
MP 治疗前应用的墨水渗透最深。微针处理在经皮输送方面具有独特的优势，因为其通道随着时间的推移显示出增加的渗透和产品的横向扩展。
