Balyan Prerna, Saini Deepika, Das Supriyo, Kumar Dhirendra, Agarwal Ajay
Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Electronics Engineering Research Institute (CSIR-CEERI) Campus, Pilani Rajasthan 333031, India.
CSIR-Central Electronics and Engineering Research Institute (CSIR-CEERI) Campus, Pilani Rajasthan 333031, India.
Biomicrofluidics. 2020 Mar 19;14(2):024103. doi: 10.1063/1.5143656. eCollection 2020 Mar.
Particle filtration and concentration have great significance in a multitude of applications. Physical filters are nearly indispensable in conventional separation processes. Similarly, microfabrication-based physical filters are gaining popularity as size-based particle sorters, separators, and prefiltration structures for microfluidics platforms. The work presented here introduces a linear combination of obstructions to provide size contrast-based particle separation. Polystyrene particles that are captured along the crossflow filters are packed in the direction of the dead-end filters. Separation of polydisperse suspension of 5 μm and 10 μm diameter polystyrene microspheres is attained with capture efficiency for larger particles as 95%. Blood suspension is used for biocharacterization of the device. A flow induced method is used to improve particle capture uniformity in a single microchannel and reduce microgap clogging to about 30%. This concept is extended to obtain semiquantification obtained by comparison of the initial particle concentration to captured-particle occupancy in a microfiltration channel.
颗粒过滤和浓缩在众多应用中具有重要意义。物理过滤器在传统分离过程中几乎不可或缺。同样，基于微加工的物理过滤器作为用于微流体平台的基于尺寸的颗粒分选器、分离器和预过滤结构正变得越来越流行。本文介绍了一种障碍物的线性组合，以提供基于尺寸对比的颗粒分离。沿错流过滤器捕获的聚苯乙烯颗粒沿死端过滤器方向堆积。实现了直径为5μm和10μm的聚苯乙烯微球多分散悬浮液的分离，较大颗粒的捕获效率为95%。血液悬浮液用于该装置的生物特性表征。采用流动诱导方法提高单个微通道中颗粒捕获的均匀性，并将微间隙堵塞减少到约30%。通过比较微滤通道中初始颗粒浓度与捕获颗粒占有率，将这一概念扩展以获得半定量结果。
