Zhao Hui-Qi, Dong Bao-Long, Zhang Min, Dong Xiao-Hua, He Yu, Chen Shi-Yong, Wu Biao, Yang Xiao-Jun
Department of General Surgery, Gansu Provincial Hospital, Lanzhou 730000, Gansu Province, China.
Department of Pathology, Gansu Provincial Hospital, Lanzhou 730000, Gansu Province, China.
World J Gastrointest Oncol. 2020 Mar 15;12(3):276-288. doi: 10.4251/wjgo.v12.i3.276.
The kinesin superfamily protein member KIF21B plays an important role in regulating mitotic progression; however, the function and mechanisms of KIF21B in cancer, particularly in hepatocellular carcinoma (HCC), are unknown.
To explore the role of KIF21B in hepatocellular carcinoma and its effect on prognosis after hepatectomy.
First, data on the differential expression of KIF21B in patients with HCC from The Cancer Genome Atlas database was analyzed. Subsequently, the expression levels of KIF21B in HCC cell lines and hepatocytes were detected by reverse transcription-polymerase chain reaction, and its biological effect on BEL-7404 cells was evaluated by knockdown. Immunohistochemical analysis was used to validate the differential expression of KIF21B in HCC tissues and adjacent normal tissues from 186 patients with HCC after hepatectomy. The Kaplan-Meier method was used to assess prognosis significance.
KIF21B expression levels were significantly higher in HCC tissues than in corresponding adjacent normal tissues. The expression levels of KIF21B in four HCC cell lines were higher than that in normal liver cells. Functional experiments showed that knockdown remarkably suppressed cell proliferation and induced apoptosis. Moreover, immunohistochemistry results are consistent with The Cancer Genome Atlas analysis, with KIF21B expression levels being increased in HCC tissues compared to adjacent normal tissues. Univariate and multivariate analyses revealed KIF21B as an independent risk factor for overall survival and disease-free survival in patients with HCC after hepatectomy.
Taken together, our results provide evidence that KIF21B plays an important role in HCC progression and may be a potential diagnostic and prognostic marker for HCC.
驱动蛋白超家族蛋白成员KIF21B在调节有丝分裂进程中发挥重要作用；然而，KIF21B在癌症，尤其是在肝细胞癌（HCC）中的功能和机制尚不清楚。
探讨KIF21B在肝细胞癌中的作用及其对肝切除术后预后的影响。
首先，分析来自癌症基因组图谱数据库的HCC患者中KIF21B的差异表达数据。随后，通过逆转录-聚合酶链反应检测KIF21B在HCC细胞系和肝细胞中的表达水平，并通过敲低来评估其对BEL-7404细胞的生物学效应。免疫组织化学分析用于验证186例肝切除术后HCC患者的HCC组织和癌旁正常组织中KIF21B的差异表达。采用Kaplan-Meier法评估预后意义。
KIF21B在HCC组织中的表达水平显著高于相应的癌旁正常组织。四种HCC细胞系中KIF21B的表达水平高于正常肝细胞。功能实验表明，敲低可显著抑制细胞增殖并诱导凋亡。此外，免疫组织化学结果与癌症基因组图谱分析一致，与癌旁正常组织相比，HCC组织中KIF21B表达水平升高。单因素和多因素分析显示，KIF21B是肝切除术后HCC患者总生存和无病生存的独立危险因素。
综上所述，我们的结果提供了证据，表明KIF21B在HCC进展中起重要作用，可能是HCC的潜在诊断和预后标志物。
