Frese Claire-Denise, Schiller Stephan
Institut für Experimentalphysik, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany.
Biomed Opt Express. 2020 Jan 2;11(2):533-553. doi: 10.1364/BOE.11.000533. eCollection 2020 Feb 1.
We demonstrate lensless imaging of three-dimensional phantoms of fluorescent nanodiamonds in solution. Magnetofluorescence imaging is employed, which relies on a dependence of the fluorescence yield on the magnetic field, and pervading the object with an inhomogeneous magnetic field. This field provides a field-free field line, which is rastered through the object. A 3D image of the object is obtained by imaging a set of 2D slices. Each 2D slice image is computed from a set of 1D projections, obtained under different projection directions, using a backprojection algorithm. Reconstructed images containing up to 36 × 36 × 8 voxels are obtained. A spatial resolution better than 2 mm is achieved in three dimensions. The approach has the potential for scalability.
我们展示了溶液中荧光纳米金刚石三维模型的无透镜成像。采用了磁荧光成像，它依赖于荧光产率对磁场的依赖性，并利用非均匀磁场穿透物体。该磁场提供了一条无场磁力线，该磁力线在物体中进行光栅扫描。通过对一组二维切片进行成像来获得物体的三维图像。每个二维切片图像由一组在不同投影方向下获得的一维投影，使用反投影算法计算得出。获得了包含多达36×36×8体素的重建图像。在三个维度上实现了优于2毫米的空间分辨率。该方法具有可扩展性的潜力。
