Yin Huadong, Zhao Jing, Han Shunshun, Cui Can, Wang Yan, Li Diyan, Zhu Qing
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130 Sichuan People's Republic of China.
3 Biotech. 2020 Apr;10(4):171. doi: 10.1007/s13205-020-2161-2. Epub 2020 Mar 17.
The Src homology 3 and cysteine-rich domain 3 gene () encodes a protein containing both a cysteine-rich domain and two Src (sarcoma) homology 3 domains (SH3). is specifically expressed in skeletal muscle and plays an important role in skeletal muscle development, but the explicit sequence and function of chicken remain unknown. In the current study, we found the full-length chicken cDNA to be 1383 bp long, with a 1092 bp open reading frame that harbors one cysteine-rich C1 domain and two SH3 domains. Tissue distribution analysis reveals chicken mRNA only in skeletal muscle among 12 chicken tissues examined by reverse transcription PCR. Both cholecystokinin octapeptide analysis and a 5-ethynyl-2'-deoxyuridine assay suggest that neither overexpression nor knockdown has any effect on the proliferation of chicken skeletal muscle satellite cells. However, knockdown significantly increases the mRNA expression of , , , and , and the protein abundance of MyHC and MyoG, whereas the opposite result is found in overexpressed cells. We conclude that the gene is expressed specifically in skeletal muscle and is a negative regulator of skeletal muscle satellite cell differentiation in chicken.
Src同源3和富含半胱氨酸结构域3基因（）编码一种既包含富含半胱氨酸结构域又包含两个Src（肉瘤）同源3结构域（SH3）的蛋白质。在骨骼肌中特异性表达，在骨骼肌发育中起重要作用，但鸡的明确序列和功能仍不清楚。在本研究中，我们发现鸡全长cDNA为1383bp，具有1092bp的开放阅读框，包含一个富含半胱氨酸的C1结构域和两个SH3结构域。组织分布分析显示，通过逆转录PCR检测的12种鸡组织中，鸡mRNA仅存在于骨骼肌中。胆囊收缩素八肽分析和5-乙炔基-2'-脱氧尿苷检测均表明，过表达或敲低对鸡骨骼肌卫星细胞的增殖均无影响。然而，敲低显著增加了、、、的mRNA表达，以及MyHC和MyoG的蛋白丰度，而在过表达细胞中则发现相反的结果。我们得出结论，基因在骨骼肌中特异性表达，是鸡骨骼肌卫星细胞分化的负调节因子。
