Zhao Pengxuan, Hou Xucheng, Yan Jingyue, Du Shi, Xue Yonger, Li Wenqing, Xiang Guangya, Dong Yizhou
Division of Pharmaceutics & Pharmacology, College of Pharmacy, The Ohio State University, Columbus, OH, 43210, United States.
School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Bioact Mater. 2020 Mar 18;5(2):358-363. doi: 10.1016/j.bioactmat.2020.03.001. eCollection 2020 Jun.
Lipid-like nanoparticles (LLNs) have been extensively explored for messenger RNA (mRNA) delivery in various biomedical applications. However, the long-term storage of these nanoparticles is still a challenge for their clinical translation. In this study, we investigated a series of conditions for the long-term storage of LLNs with encapsulation of mRNA. We evaluated the stability of LLNs with different concentrations of cryoprotectants (sucrose, trehalose or mannitol) under the conditions of freezing or lyophilization processes. Through and mRNA delivery studies, we identified the optimal storage condition, and found that the addition with 5% (w/v) sucrose or trehalose to LLNs could remain their mRNA delivery efficiency for at least three months in the liquid nitrogen storage condition.
脂质样纳米颗粒（LLNs）已在各种生物医学应用中被广泛研究用于信使核糖核酸（mRNA）递送。然而，这些纳米颗粒的长期储存仍是其临床转化面临的一个挑战。在本研究中，我们研究了一系列用于封装mRNA的LLNs长期储存的条件。我们评估了在冷冻或冻干过程条件下，不同浓度的冷冻保护剂（蔗糖、海藻糖或甘露醇）对LLNs稳定性的影响。通过mRNA递送研究，我们确定了最佳储存条件，并发现向LLNs中添加5%（w/v）的蔗糖或海藻糖，在液氮储存条件下可使它们的mRNA递送效率至少保持三个月。
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