Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul, 03080, South Korea.
Institute on Aging, Seoul National University, Seoul, South Korea.
Clin Oral Investig. 2020 Nov;24(11):3961-3970. doi: 10.1007/s00784-020-03262-z. Epub 2020 Mar 23.
To investigate the viscosity values of mixtures of hyaluronic acids with different molecular weights and the effects of these mixtures on the enzymatic activities of lysozyme and peroxidase.
Mixtures of high molecular weight (1 or 2 MDa) and low molecular weight (10 or 100 kDa) hyaluronic acids at different concentrations were used for viscosity measurements. Hyaluronic acid mixtures showing viscosity values similar to those of human whole saliva were used for enzyme experiments in solution and on hydroxyapatite surface. Hen egg-white lysozyme, bovine lactoperoxidase, and human whole saliva were used as enzyme sources. Lysozyme activity was measured by hydrolysis of fluorescein-labeled Micrococcus lysodeikticus. Peroxidase activity was measured by oxidation of fluorogenic 2',7'-dichlorofluorescein to fluorescing 2',7'-dichlorofluorescein.
The mixtures of 1 MDa (0.5 mg/mL) or 2 MDa (0.2 mg/mL) hyaluronic acid with 10 kDa (2.0 mg/mL) or 100 kDa (0.1 mg/mL) hyaluronic acid had viscosity values similar to those of human whole saliva at shear rates, reflecting normal oral functions. Compared with single molecular weight hyaluronic acids, these mixtures showed viscosity values more similar to those of human whole saliva. The mixtures inhibited lysozyme and peroxidase activities on the hydroxyapatite surfaces; however, the degree of inhibition did not differ from that of hyaluronic acid of 1 or 2 MDa only.
Compared with single molecular weight hyaluronic acids, hyaluronic acid mixtures showed viscosity values more similar to those of human whole saliva, without additional inhibitory effects on lysozyme and peroxidase activities.
Hyaluronic acid mixtures offer distinct advantages for the development of saliva substitutes.
研究不同分子量的透明质酸混合物的黏度值以及这些混合物对溶菌酶和过氧化物酶酶活性的影响。
使用不同浓度的高分子量（1 或 2 MDa）和低分子量（10 或 100 kDa）透明质酸混合物进行黏度测量。用于酶实验的透明质酸混合物具有与人类全唾液相似的黏度值，可在溶液中和羟磷灰石表面进行。使用鸡卵清溶菌酶、牛乳过氧化物酶和人类全唾液作为酶源。通过水解荧光素标记的微球菌溶菌酶来测量溶菌酶活性。通过氧化荧光性 2'，7'-二氯荧光素来测量过氧化物酶活性，生成荧光性 2'，7'-二氯荧光素。
1 MDa（0.5 mg/mL）或 2 MDa（0.2 mg/mL）透明质酸与 10 kDa（2.0 mg/mL）或 100 kDa（0.1 mg/mL）透明质酸的混合物在剪切速率下具有与人类全唾液相似的黏度值，反映了正常的口腔功能。与单一分子量的透明质酸相比，这些混合物的黏度值更接近人类全唾液。这些混合物抑制了羟磷灰石表面上的溶菌酶和过氧化物酶活性；然而，抑制程度与仅 1 或 2 MDa 的透明质酸没有差异。
与单一分子量的透明质酸相比，透明质酸混合物的黏度值更接近人类全唾液，并且对溶菌酶和过氧化物酶活性没有额外的抑制作用。
透明质酸混合物为开发唾液替代品提供了明显的优势。
