Department of Dentistry, School of Dentistry, Seoul National University, Seoul, Korea.
Oral Dis. 2011 Sep;17(6):577-83. doi: 10.1111/j.1601-0825.2011.01807.x. Epub 2011 Apr 8.
To investigate the fungistatic and fungicidal activity of hyaluronic acid (HA) and the influences of HA on the anticandidal activities of lysozyme and the peroxidase system.
HA, hen egg-white lysozyme, and the bovine lactoperoxidase system were used. Candida albicans ATCC 10231, 18804, and 11006 strains were used in the experiments. The fungistatic activity of HA was determined by measuring the optical densities of the cultures. The candidacidal activity of HA and the influences of HA on the candidacidal activities of lysozyme and the peroxidase system were determined by comparing the numbers of colony-forming units.
Hyaluronic acid displayed inhibitory effects on the growth of C. albicans, and the inhibitory effects were proportional to HA concentration. HA did not have any measurable candidacidal activity. HA showed inhibitory effects on the candidacidal activities of lysozyme, and the peroxidase system that was proportional to HA concentration. HA at 1.0-2.0 mg ml(-1) almost completely inhibited the candidacidal activities of lysozyme and the peroxidase system.
Hyaluronic acid possesses fungistatic activity but no candidacidal activity. HA showed inhibitory effects on the candidacidal activities of lysozyme and the peroxidase system.
研究透明质酸（HA）的抑菌和杀菌活性，以及 HA 对溶菌酶和过氧化物酶系统抗真菌活性的影响。
使用 HA、鸡卵清溶菌酶和牛乳过氧化物酶系统。实验中使用了白色念珠菌 ATCC 10231、18804 和 11006 株。通过测量培养物的光密度来确定 HA 的抑菌活性。通过比较菌落形成单位数来确定 HA 的杀菌活性以及 HA 对溶菌酶和过氧化物酶系统杀菌活性的影响。
透明质酸对白色念珠菌的生长表现出抑制作用，抑制作用与 HA 浓度成正比。HA 没有任何可测量的杀菌活性。HA 对溶菌酶和过氧化物酶系统的杀菌活性表现出抑制作用，抑制作用与 HA 浓度成正比。HA 在 1.0-2.0 mg/ml 时几乎完全抑制了溶菌酶和过氧化物酶系统的杀菌活性。
透明质酸具有抑菌活性，但没有杀菌活性。HA 对溶菌酶和过氧化物酶系统的杀菌活性有抑制作用。
