利用疏水型深共晶溶剂进行绿色、快速和简单的分散液液微萃取方法，用于分析加标面粉样品中的叶酸，然后进行液相色谱测定。
Green, fast and simple dispersive liquid-liquid microextraction method by using hydrophobic deep eutectic solvent for analysis of folic acid in fortified flour samples before liquid chromatography determination.
机构信息
Research Group of Food, Halal and Agricultural Products, Research Department of Food Technology and Agricultural Products, Standard Research Institute (SRI), Karaj P.O. Box 31745-139, Iran.
Research Group of Food Toxicology, Research Department of Food Technology and Agricultural Products, Standard Research Institute (SRI), Karaj P.O. Box 31745-139, Iran.
出版信息
Food Chem. 2020 Aug 1;320:126486. doi: 10.1016/j.foodchem.2020.126486. Epub 2020 Feb 25.
A fast, simple, sensitive, and efficient vortex-assisted dispersive liquid-liquid microextraction method (VA-DES-DLME) was developed based on hydrophobic deep eutectic solvent for extraction of folic acid from flour samples followed by HPLC determination. Hydrophobic DES was prepared by mixing amylalcohol as a hydrogen bond donor and methyltriocthylammonium chloride as a hydrogen bond acceptor. Factors affecting DLLME were considered and optimized. In optimal conditions, the calibration curve was linear in the range 5.0-500 ng g with a correlation coefficient higher than 0.99. The limit of detection (LOD) and the limit of quantification (LOQ) were 1.0 and 3.0 ng g, respectively. The intra-day and inter-day precision at concentrations of 10 and 100 ng g were less than 8%. Finally, application of the proposed procedure was investigated by folic acid analysis in flour samples and the relative recoveries obtained were acceptable (Recovery ≥ 90%).
一种基于疏水深共晶溶剂的快速、简单、灵敏、高效的涡旋辅助分散液液微萃取方法（VA-DES-DLME）被开发出来，用于从面粉样品中萃取叶酸，然后用 HPLC 测定。疏水 DES 是通过混合作为氢键供体的戊醇和作为氢键受体的甲基三辛基氯化铵制备的。考虑并优化了影响 DLLME 的因素。在最佳条件下，校准曲线在 5.0-500ng g 范围内呈线性，相关系数高于 0.99。检测限（LOD）和定量限（LOQ）分别为 1.0 和 3.0ng g。在 10 和 100ng g 浓度下，日内和日间精密度小于 8%。最后，通过在面粉样品中分析叶酸来研究所提出的方法的应用，得到的相对回收率是可以接受的（回收率≥90%）。
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