通过在CM-东丽珠上进行一步色谱法分离赤坂扇贝和兔肌钙蛋白成分。

Separation of Akazara scallop and rabbit troponin components by a single-step chromatography on CM-Toyopearl.

作者信息

Ojima T, Nishita K

机构信息

Department of Chemistry, Faculty of Fisheries, Hokkaido University.

出版信息

J Biochem. 1988 Jul;104(1):9-11. doi: 10.1093/oxfordjournals.jbchem.a122429.

DOI:10.1093/oxfordjournals.jbchem.a122429

PMID:3220835

Abstract

Three components of Akazara scallop (Chlamys nipponensis akazara) troponin were well separated from each other by a single-step chromatography on CM-Toyopearl, although they were hardly separated on DEAE-Sephadex A-25. Moreover, by means of this CM-chromatography, the troponin components of rabbit were also readily separated with high purities and in high yields. The components thus separated were readily reconstituted and the Ca2+ regulatory function was fully recovered.

摘要

通过在CM - Toyopearl上进行一步层析，赤坂扇贝（Chlamys nipponensis akazara）肌钙蛋白的三个组分能够很好地相互分离，尽管它们在DEAE - Sephadex A - 25上几乎无法分离。此外，通过这种CM层析法，兔肌钙蛋白组分也能很容易地以高纯度和高产量分离出来。如此分离得到的组分很容易重新组装，并且Ca2 +调节功能得以完全恢复。