Inoue A, Ojima T, Nishita K
Department of Chemistry, Faculty of Fisheries, Hokkaido University.
J Biochem. 1996 Oct;120(4):834-7. doi: 10.1093/oxfordjournals.jbchem.a021487.
A cDNA clone encoding troponin T of Akazara scallop (Chlamys nipponensis akazara) striated adductor muscle has been isolated and sequenced. The complete sequence deduced consists of 314 amino acid residues with a molecular weight of 37,206. Akazara scallop troponin T contains 55 amino acid residues more and 82 residues fewer than rabbit skeletal muscle troponin T and Drosophila melanogaster troponin T, respectively, showing almost the lowest sequence homology with rabbit troponin T (26%) but the highest homology with Drosophila troponin T (33%). Further, high sequence homology was seen in the functional regions: residues 33-120 and 174-227, corresponding respectively to residues 71-158 and 197-250 of rabbit troponin T (tropomyosin-binding regions); and residues 200-204, corresponding to 223 227 of rabbit troponin T (troponin I-binding region). In residues 1-70 (tropomyosin-binding region), however, only six residues are identical with rabbit troponin T.
已分离并测序了一个编码赤坂扇贝（Chlamys nipponensis akazara）横纹肌中肌钙蛋白T的cDNA克隆。推导的完整序列由314个氨基酸残基组成，分子量为37,206。赤坂扇贝肌钙蛋白T分别比兔骨骼肌肌钙蛋白T和黑腹果蝇肌钙蛋白T多55个氨基酸残基和少82个残基，与兔肌钙蛋白T的序列同源性几乎最低（26%），但与果蝇肌钙蛋白T的同源性最高（33%）。此外，在功能区域观察到高序列同源性：第33 - 120位残基和第174 - 227位残基，分别对应于兔肌钙蛋白T的第71 - 158位残基和第197 - 250位残基（原肌球蛋白结合区域）；以及第200 - 204位残基，对应于兔肌钙蛋白T的第223 - 227位残基（肌钙蛋白I结合区域）。然而，在第1 - 70位残基（原肌球蛋白结合区域）中，只有六个残基与兔肌钙蛋白T相同。
