Sato Y, Fujiwara K
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo.
J Biochem. 1988 Jul;104(1):98-101. doi: 10.1093/oxfordjournals.jbchem.a122431.
Hepatic ornithine decarboxylase activity in rats increased 2 h after partial hepatectomy, showing two peaks at 4 and 10 h. When the rats received 1,3-diaminopropane (DAP) from 0 to 4 h or from 6 to 10 h, this increase was suppressed at 6 or 12 h, respectively, whereas hepatic spermidine N1-acetyltransferase activity was enhanced by DAP administration at 6 as well as 12 h, though the levels at 12 h were one-fifth of those at 6 h. An increase in hepatic DNA synthesis at 22 h did not occur in the rats given DAP from 6 to 10 h. It recovered after administration of putrescine, but not that of spermidine. In contrast, such an inhibition was not seen in the rats given DAP from 0 to 4 h; it occurred when quinacrine, a polyamine oxidase inhibitor, was concomitantly dosed, and disappeared with further addition of putrescine. Hepatic DNA synthesis changed in close association with hepatic putrescine content irrespective of spermidine and spermine contents in these rats. Putrescine may be essential for liver regeneration after partial hepatectomy, and can be produced in sufficient quantity to support hepatic DNA synthesis by the compensatory route of spermidine acetylation and oxidation when ornithine decarboxylase activity is suppressed at an early stage.
大鼠部分肝切除术后2小时肝鸟氨酸脱羧酶活性增加，在4小时和10小时出现两个峰值。当大鼠在0至4小时或6至10小时接受1,3 - 二氨基丙烷（DAP）时，这种增加分别在6小时或12小时受到抑制，而肝亚精胺N1 - 乙酰转移酶活性在6小时和12小时时通过给予DAP而增强，尽管12小时时的水平是6小时时的五分之一。在6至10小时给予DAP的大鼠中，22小时时肝DNA合成未增加。给予腐胺后恢复，但给予亚精胺后未恢复。相反，在0至4小时给予DAP的大鼠中未观察到这种抑制；当同时给予多胺氧化酶抑制剂奎纳克林时发生抑制，进一步添加腐胺后抑制消失。在这些大鼠中，肝DNA合成的变化与肝腐胺含量密切相关而与亚精胺和精胺含量无关。腐胺可能是部分肝切除术后肝脏再生所必需的，并且当鸟氨酸脱羧酶活性在早期受到抑制时，可以通过亚精胺乙酰化和氧化的补偿途径产生足够数量的腐胺来支持肝DNA合成。
