Korbelár P, Vacík J, Dylevský I
Orthopaedic Clinic, Paediatric Faculty, Charles University, Prague, Czechoslovakia.
J Biomed Mater Res. 1988 Sep;22(9):751-62. doi: 10.1002/jbm.820220902.
The present study deals with the application and possibilities of insoluble hydrophilic gels (poly(2-hydroxyethyl methacrylate] as substitutes of bone tissue experimentally. Their biocompatibility is examined with regard to the porous qualities of the implant and to its chemical structure, and their behavior in the cancellous and compact bone is evaluated. It was found that the modifications of hydrogels used in the experiment are biocompatible, with the compatibility increasing in proportion to increasing porosity. The nonporous and microporous hydrogels are not compatible and are demarcated. The sintered macroporous gel is surrounded by a thin fibrin membrane. By adding methacrylic acid to the hydrogel surface, adhesion increases markedly. Marked destruction also appears in the polymer especially in the cancellous bone. By an active destruction of the polymer, no direct phagocytosis can be proved. Upon breakdown of the implant in the compact bone the activity of the macrophages is delayed. When the gel without methacrylic acid is used alone, destruction does not occur even after 193 days. When methacrylic acid is added to the polymer surface, destruction does occur and the implant is filled only by bone trabeculae.
本研究探讨了不溶性亲水性凝胶（聚甲基丙烯酸羟乙酯）作为骨组织替代物的实验应用及可能性。针对植入物的多孔性质及其化学结构，对其生物相容性进行了检测，并评估了它们在松质骨和密质骨中的表现。结果发现，实验中使用的水凝胶改性物具有生物相容性，且相容性随孔隙率增加而提高。无孔和微孔水凝胶不相容且有分界。烧结大孔凝胶被一层薄的纤维蛋白膜包围。通过在水凝胶表面添加甲基丙烯酸，黏附力显著增加。聚合物中也出现明显破坏，尤其是在松质骨中。通过聚合物的主动破坏，未证实有直接吞噬作用。当植入物在密质骨中分解时，巨噬细胞的活性会延迟。单独使用不含甲基丙烯酸的凝胶时，即使193天后也不会发生破坏。当在聚合物表面添加甲基丙烯酸时，确实会发生破坏，且植入物仅被骨小梁填充。
