School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Microbiology (Reading). 2020 Jul;166(7):617-623. doi: 10.1099/mic.0.000897.
The oleaginous fungus can synthesize a variety of polyunsaturated fatty acids, which are used extensively in industry for the production of arachidonic acid (AA). NADPH is the limiting factor and critical reducing agent in lipid biosynthesis. In the folate cycle, methylenetetrahydrofolate dehydrogenase (MTHFDL) catalyzes the conversion of methylene tetrahydrofolate into 10-formyl-tetrahydrofolate with the reduction of NADP to NADPH. MTHFDL RNAi was used to investigate the role of the folate cycle in lipogenesis. Gene knockdown decreased the transcript levels of MTHFDL by about 50 % and attenuated cell fatty acid synthesis. The observation of decreased NADPH levels and downregulated NADPH-producing genes in response to MTHFDL RNAi indicates a novel aspect of the NADPH regulatory mechanism. Thus, our study demonstrates that MTHFDL plays key role in the mediation of NADPH in lipogenesis in .
油脂真菌能够合成多种多不饱和脂肪酸，这些脂肪酸被广泛应用于工业生产花生四烯酸（AA）。NADPH 是脂质生物合成中的限制因素和关键还原剂。在叶酸循环中，亚甲基四氢叶酸脱氢酶（MTHFDL）催化亚甲基四氢叶酸转化为 10-甲酰基四氢叶酸，同时将 NADP 还原为 NADPH。我们使用 MTHFDL RNAi 来研究叶酸循环在脂肪生成中的作用。基因敲低使 MTHFDL 的转录水平降低了约 50%，并减弱了细胞脂肪酸的合成。观察到 MTHFDL RNAi 会导致 NADPH 水平下降和 NADPH 生成基因下调，这表明 NADPH 调节机制的一个新方面。因此，我们的研究表明，MTHFDL 在油脂真菌中脂肪生成的 NADPH 介导中发挥关键作用。
