从紫色小单孢菌培养滤液中分离庆大霉素C化合物。

Isolation of gentamicin C compounds from culture filtrates of Micromonospora purpurea.

作者信息

Jork H, Kunz F R

机构信息

FB. Pharmazie und Biologische Chemie, Universität des Saarlandes, F.R.G.

出版信息

J Chromatogr. 1988 Oct 19;450(1):115-9. doi: 10.1016/s0021-9673(00)90722-2.

DOI:10.1016/s0021-9673(00)90722-2

PMID:3220920

Abstract

A liquid chromatographic method was developed for the isolation of gentamicin C compounds from commercial fermentation products in order to monitor health hazards (oto- and nephrotoxicity). Chromatography was carried out on silica gel 60 (15-40 microns) with a medium-pressure chromatographic system, employing methanol-25% ammonia solution (85:15, v/v) and methanol-chloroform-25% ammonia solution (20:10:5, v/v) as mobile phases. The eluted fractions were neutralized with 1.0 M hydrochloric acid, concentrated in vacuo and desalted by gel filtration. It was possible to demonstrate by 1H NMR spectroscopy and high-performance liquid and thin-layer chromatography that the separated fractions contained components C1, C1a and C2 in purities of more than 95%.

摘要

为监测健康危害（耳毒性和肾毒性），开发了一种液相色谱法，用于从商业发酵产品中分离庆大霉素C化合物。使用中压色谱系统，在硅胶60（15 - 40微米）上进行色谱分离，采用甲醇 - 25%氨溶液（85:15，v/v）和甲醇 - 氯仿 - 25%氨溶液（20:10:5，v/v）作为流动相。洗脱馏分用1.0 M盐酸中和，真空浓缩并通过凝胶过滤脱盐。通过1H NMR光谱、高效液相色谱和薄层色谱可以证明，分离出的馏分含有纯度超过95%的C1、C1a和C2组分。