通过克雷格分配法将庆大霉素C复合物分离为五个组分。

Separation of gentamicin C-complex into five components by Craig distribution.

作者信息

Byrne K M, Kershner A S, Maehr H, Marquez J A, Schaffner C P

出版信息

J Chromatogr. 1977 Jan 21;131:191-203. doi: 10.1016/s0021-9673(00)80933-4.

Abstract

A gentamicin C-complex preparation was separated preparatively into five components by Craig distribution. Gentamicins C1, C2, and C1a were the major components, whereas components C2a and C2b represented only 4% of the total C-complex mixture. Paper chromatographic analysis showed that the gentamicin C2b separated by Craig distribution was identical with a gentamicin isolated from Micromonospora purpurea var. JI-33 fermentation broth and identified as 6'-N-methylgentamicin C1a. Similarly, component C2a was identical with a previously separated gentamicin component tentatively identified as the 6'-C epimer of gentamicin C2.

摘要

通过克雷格分配法将庆大霉素C复合物制剂制备性地分离为五个组分。庆大霉素C1、C2和C1a是主要组分，而组分C2a和C2b仅占总C复合物混合物的4%。纸色谱分析表明，通过克雷格分配法分离得到的庆大霉素C2b与从紫色小单孢菌JI-33发酵液中分离得到并鉴定为6'-N-甲基庆大霉素C1a的庆大霉素相同。同样，组分C2a与先前分离的一种庆大霉素组分相同，该组分初步鉴定为庆大霉素C2的6'-C差向异构体。