Xue Lu-Lu, Wang Fang, Niu Rui-Ze, Tan Ya-Xin, Liu Jia, Jin Yuan, Ma Zheng, Zhang Zi-Bin, Jiang Ya, Chen Li, Xia Qing-Jie, Chen Jun-Jie, Wang Ting-Hua, Xiong Liu-Lin
Department of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou Province; Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan Province, China.
Department of Science and Technology, Kunming Medical University, Kunming, Yunnan Province, China.
Neural Regen Res. 2020 Sep;15(9):1662-1670. doi: 10.4103/1673-5374.276359.
Neonatal hypoxic-ischemic encephalopathy is a serious neurological disease, often resulting in long-term neurodevelopmental disorders among surviving children. However, whether these neurodevelopmental issues can be passed to offspring remains unclear. The right common carotid artery of 7-day-old parental-generation rats was subjected to permanent ligation using a vessel electrocoagulator. Neonatal hypoxic-ischemic rat models were established by subjecting the rats to 8% O-92% N for 2 hours. The results showed that 24 hours after hypoxia and ischemia, pathological damage, cerebral atrophy, liquefaction, and impairment were found, and Zea-Longa scores were significantly increased. The parental-generation rats were propagated at 3 months old, and offspring were obtained. No changes in the overall brain structures of these offspring rats were identified by magnetic resonance imaging. However, the escape latency was longer and the number of platform crossings was reduced among these offspring compared with normal rats. These results indicated that the offspring of hypoxic-ischemic encephalopathy model rats displayed cognitive impairments in learning and memory. This study was approved by the Animal Care & Welfare Committee of Kunming Medical University, China in 2018 (approval No. kmmu2019072).
新生儿缺氧缺血性脑病是一种严重的神经系统疾病，常导致存活儿童出现长期神经发育障碍。然而，这些神经发育问题是否会遗传给后代仍不清楚。使用血管电凝器对7日龄亲代大鼠的右侧颈总动脉进行永久性结扎。通过将大鼠置于8%氧气-92%氮气环境中2小时建立新生儿缺氧缺血大鼠模型。结果显示，缺氧缺血24小时后，发现有病理损伤、脑萎缩、液化和功能障碍，且Zea-Longa评分显著升高。亲代大鼠在3月龄时繁殖，获得子代。磁共振成像未发现这些子代大鼠的整体脑结构有变化。然而，与正常大鼠相比，这些子代的逃避潜伏期更长，穿越平台的次数减少。这些结果表明，缺氧缺血性脑病模型大鼠的子代在学习和记忆方面存在认知障碍。本研究于2018年获得中国昆明医科大学动物保护与福利委员会批准（批准号：kmmu2019072）。
